Cloning and Sequencing of the Glu B-1 Promoter from Rice

doi:10.3321/j.issn:1000-7091.2006.06.004

Abstract

Abstract: The GluB_1 promoter was amplified from Oryza sativa genome by polymerase chain reaction and cloned into pGEM-T Easy vector.Sequence analysis showed that the obtained promoter fragment 2293 nucleotides and showed homology of 99.2% with the reported Glu B-promoter.Also,the obtained promoter fragment includes the three cis-regulatory elements,ACGT-motif,AACA-motif and GCN4-motif,which are necessary to mediate endosperm expression of the GluB_1 glutelin gene.

Key words: Oryza sativa, GluB_1 gene, Promoter, Glutelin

CLC Number:

S511.035.3

WANG Wei-quan, LIU Li, WANG Jing-xue. Cloning and Sequencing of the Glu B-1 Promoter from Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(6): 16-18. doi: 10.3321/j.issn:1000-7091.2006.06.004.

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