Genetic Transformation of Carrot Mediated by Agrobacterium and Study on Culture Management in Transgenic Carrot

doi:10.3321/j.issn:1000-7091.2006.06.002

Abstract

Abstract: Seeds of carrots were selected for transformation via Agrobacterium to obtain transgenic plants.The results showed that frequency of the resistant callus and shoot regeneration in different concentration of Kanamycin(Kan) reached 52%.Carbenicillin(Carb) 500 mg/L completely inhibited the growth of Agrobacterium and promoted the growth of the resistant shoots.Moderate concentration of Cefotamine(Cef) could promote the growth of root.The resistance of plants covered with plastic membrane and gradually uncovered grew well and the survival rate enhanced 35%.Compared plants in the fields with those in the greenhouse,the death rate of carrot seedlings in the greenhouse reduced 49.5% in low temperature vernalization.PCR analysis for 7 transgenic plants in T-1 generation showed two lines of them were close to 1:1 and other two lines almost close to 1:3.

Key words: Carrots, Transgenic transformation, Vernalization, Agrobacterium mediated

CLC Number:

Q789

GUO Rong, LI Xia, ZHANG Jin-wen, LI Xing-tao, YANG Hui, LIU Ling. Genetic Transformation of Carrot Mediated by Agrobacterium and Study on Culture Management in Transgenic Carrot[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(6): 6-10. doi: 10.3321/j.issn:1000-7091.2006.06.002.

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