Construction of Expression Vector of ACC Synthase Gene from Persimmon for RNA Interfering

doi:10.3321/j.issn:1000-7091.2007.01.017

Abstract

Abstract: According to the restriction enzyme site of the expression vector and cloning sequence, two pairs of primers containing restriction enzyme site were desigined and used to amplify sequenced plasmid. PCR products and the plasmid pSMAK311 were digested by the corresponding restricted enzymes respectively, and linked directionally. Then the shRNA expression vector can be designed. After sequencing transcription, the hairpin mRNA obtained due to the inverted-repeat DNA fragment. The constructed expression vector was transformed into Agrobacterium EHA101 for the following-up research.

Key words: Persimmon, ACC synthase, RNA interference, Plant expression vector

CLC Number:

S665.2

TANG Xia, ZHOU Zhi-ping, ZHAO Cong-zhi, MA Jun-lian, ZHANG Zi-de. Construction of Expression Vector of ACC Synthase Gene from Persimmon for RNA Interfering[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(1): 73-77. doi: 10.3321/j.issn:1000-7091.2007.01.017.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.3321/j.issn:1000-7091.2007.01.017

http://www.hbnxb.net/EN/Y2007/V22/I1/73

[1]	WANG Xiaoya, BU Ruifang, HU Haiyan, LONG Qiang, SUN Lianxuan, QI Lu, LIU Zheng, LI Xiaoyao, LI Chengwei. *Cloning and Function Analysis of TaJIP2* Gene Negatively Regulating Growth, Maturity and Yield in Triticum aestivum L.** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(6): 35-44.
[2]	XING Man, HONG Bo, ZHANG Zerong, ZHANG Xiyu, CHEN Tuo, WU Ningrou, GUAN Mei, WU Xianmeng, GUAN Chunyun, XIONG Xinghua. Seed-specific Expression of BnGPAT9 Gene Controlled by Napin Promoter in Arabidopsis thaliana [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(4): 1-7.
[3]	WANG Shuang, YANG Rui, BAI Wei. Construction and Expression Analysis of RNA Interference Vector of WRKY Transcriptional Factor OsWRKY76 in Rice [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(6): 9-13.
[4]	HAN Xiaodong, GUO Rongqi, GAO Yang, YU Xiumin, SU Jie, ZHANG Ziyi, LI Guojing, WANG Ruigang. *The Responses of VHA-c1* Gene in Arabidopsis thaliana to Abiotic Stresses** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(4): 62-66.
[5]	LIU Yijie, CHEN Silong, CHENG Zengshu, WANG Jin, SONG Yahui, HAO Junhui, ZHANG Pengjuan, LI Yurong. Vector Construction to Overexpress AhPLDα Genes from Peanut and Genetic Transformation into Arabidopsis thaliana [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(6): 31-38.
[6]	MA Tong-hui. Effect of Different Branch Angles on Nutrition of Young Persimmon [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 179-183.
[7]	NI Zhi-yong, YU Yue-hua, LIU Chao, REN Yan-ping, CHEN Quan-jia, QU Yan-ying. *Isolation of gma-miR160o* Promoter from Glycine max and Construction of Its Plant Expression Vectors** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(1): 19-23.
[8]	TIAN Li-li, NIU Liang. Construction of RNA Interference Vector of Coat Protein Gene of Grapevine Fanleaf Virus and the Effect Evaluation in Transgenic Nicotiana benthamiana [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(S1): 1-5.
[9]	GAO Le, ZHAI Rui, DING Xue-ni, LI Kai, ZHANG Hong-yun, WANG Tao, ZHI Hai-jian. Cloning of Interference Fragment of Eukaryotic Translation Initiation Factor 4E of Soybean and Construction of Its RNAi Vector [J]. journal1, 2014, 29(4): 1-6.
[10]	ZHANG Shuai, ZHANG Ming, KOU Tian-shu, MA Jun-lian, TANG Xia, ZHANG Zi-de. Cloning and Sequence Analysis of a DNA Encoding ACC Synthase Gene from Ficus carica [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(6): 34-37.
[11]	LI Zhen, ZHANG Gui-lin, WANG Qing-guo, LIU Xu, LI Hai-qing, YAO Fang-yin, LIU Wei. Construction of RNA Interference Vectors for Rice Black-streaked Dwarf Virus and Genetic Transformation [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(4): 144-148.
[12]	ZHU Yun, FAN Na-na, YU Yao-hua, ZHANG Bai-shun, YANG Yu, CHAO Xiang-jian, GUO Bao-tai, JIN Li-ping, GUO Xiao, LI Guang-eun. RNA Interference Analysis of Receptor-like Kinase Gene （StRLK） in Potato [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(4): 18-22.
[13]	SUN Jing-wen, CHENG Ming-fang, LIANG Guo-qing, WANG Xiu-bin, ZHOU Wei. *Cloning of AmPCS* Gene from Amaranthus mangostanus and Construction of Its Plant Expression Vectors** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(2): 44-49.
[14]	LI Ya-dong, HE Jin-gang. *The Diseases Resistance Analysis of OsBRR1,A Putative Rice Receptor Like Kinase* [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(4): 27-31.
[15]	HU Bao-gang, LIU Li, JIAO Ding-liang. Cloning and Sequence Analysis of ACC SynthaseGene in Subgynoecious Watermelon [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(1): 67-71.

Please choose a citation manager

Content to export