Differential Expression of Genes in Wheat Near Isogenic Line TcLr41

doi:10.7668/hbnxb.2008.05.027

Abstract

Abstract: The objective of this paper is to study the resistant genes expression profile of wheat leaves and the wheatresistant mechanism to the pathogen at the molecular level.cDNA-AFLP analysiswas carried out between on Thatcher and its near isogenic line T cLr41resistant to wheat leaf rust to study the differential expression related toLr41.A total of 180 primer combinat ions were screened and sixty-one of them showed the ability to produce polymorphic fragments between TcLr41and Thatcher.Five of the 61 primer combinations that were P-AC/M-CAA,P-AG/M-CAT,P-AG/M-CCC,P-CC/M-CCA and P-GA/M-CAA amplified specific bands with TcLr41respectively Four repeatably differential expression fragments were reclaimed from the polyacrylamide gels,reamplified,cloned and sequenced.After blasting analysis shouled that one of the fragments was high homology with the protein encoded by wheat leaf rust resistant gene Lr1.No similar sequences were found in GenBank for the other three fragments.The results will facilitate researches on the resistance mechanism and the cloning of TcLr41.

Key words: Wheat leaf rust, Resistance gene, cDNA-AFLP, Lr41<.i>

CLC Number:

S432.1

LI Xing, LI Ya-ning, LIU Da-qun, YANG Wen-xiang. Differential Expression of Genes in Wheat Near Isogenic Line TcLr41[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(5): 122-126. doi: 10.7668/hbnxb.2008.05.027.

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