Polymorphism and Bioinformatics Analysis of SLA-DQA Gene CDs in Yantai Black Pig

doi:10.7668/hbnxb.2016.05.013

Abstract

Abstract: The PCR-SSCP,cloning sequencing and bioinformatics methods were used to explore the Yantai black pig SLA-DQA gene coding polymorphisms,structural prediction and functional characteristics of the protein.The results showed that SLA-DQA gene exon 2,exon 3 and 4 were detected 4,6,4 alleles and 5,8,7 genotypes,respectively,and three new alleles were found for the first time.The SLA-DQA coding region encoded 255 amino acids,32 nucleotide mutations and 13 amino acid mutations were found,and exon 2 showed the most polymorphism.Protein prediction results showed that random coil and extended chain accounted for the highest level,alpha helix distribution concentrated,beta sheet appeared the least.The putative result of SLA-DQA gene protein function showed that the odds of energy metabolism,structural protein and growth factor were higher.These results could provide the theory foundation for the application of SLA-DQA gene to pig disease resistance molecular breeding.

Key words: Yantai black pig, SLA-DQA gene, Coding region, Polymorphism, Protein structure

CLC Number:

S828
Q78

HUANG Xiaoyu, YUAN Junhu, YANG Qiaoli, MA Yanping, GUN Shuangbao. Polymorphism and Bioinformatics Analysis of SLA-DQA Gene CDs in Yantai Black Pig[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(5): 86-93. doi: 10.7668/hbnxb.2016.05.013.

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