Identification of RAPD Molecular Markers Linked to CMV Resistance Gene in Squash

doi:10.3321/j.issn:1000-7091.2007.01.021

Abstract

Abstract: The study was carried out using a F₂ population from a cross between resistant inbred line and susceptible inbred line. Bulked segregant analysis (BSA) was applied to identify RAPD markers linked to the resistance to CMV in squash. 520 random primers and 310 double primers with arbitrary sequences were chosen for RAPD amplification. It was found that the polymorphism between the R-bulk and S-bulk could be detected by 6% and 5% of the primers as well as two RAPD markers S439₁₄₀₀ and S19+S345₆₀₀ were linked to the resistance gene of K01 with the overcross value of 7.5% and 11.8%, genetic distance of 7.1 and 11.7 cM.

Key words: Squash, CMV, RAPD, Molecular marker

CLC Number:

S642.1

ZHAO Fu-kuan, ZHOU Hui, GAO Xia-hong, LIN Cheng. Identification of RAPD Molecular Markers Linked to CMV Resistance Gene in Squash[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(1): 90-94. doi: 10.3321/j.issn:1000-7091.2007.01.021.

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http://www.hbnxb.net/EN/Y2007/V22/I1/90

References

[1] 赵福宽, 高遐虹, 程继鸿, 等. 茄子抗冷细胞变异体的RAPD分析及自交一代株系的抗冷性鉴定[J]. 华北农学报, 2003, 18(1): 17-20.
[2] 朱华武. 茄子青枯病抗性鉴定和抗性基因的RAPD标记研究[D]. 长沙: 湖南农业大学, 2003.
[3] 苏爱莲. 一个新的抗小麦白粉病基因的RAPD标记及在育种中进行标记辅助选择的研究[D]. 兰州: 甘肃农业大学, 2000.
[4] 曲娟娟. 大豆疫霉根腐病抗病基因的RAPD标记研究[D]. 哈尔滨: 东北农业大学, 2001.
[5] 许莉萍. 甘蔗抗感黑穗病池的构建和抗病基因分子标记[D]. 福州: 福建农林大学, 2000.
[6] 张翠茹. 小麦抗叶锈基因Lr38的RAPD分子标记[D]. 保定: 河北农业大学, 2001.
[7] 陈道明. 小麦耐盐基因的RAPD标记及其克隆[D]. 北京: 中国农业科学院, 2000.
[8] 徐炎. 中国野生葡萄抗白腐病、炭疽病基因RAPD标记及其克隆[D]. 扬陵: 西北农林科技大学, 2000.
[9] 杨瑞. 番茄耐冷性PAPD分子标记的筛选及特异片段的克隆[D]. 乌鲁木齐: 新疆农业大学, 2004.
[10] 许红星, 刘红彦, 李锁平. 小麦抗白粉病基因Pm6的RAPD标记和SCAB标记[J]. 河南农业科学, 2004, 4: 25-28.
[11] 刘志勇, 孙其信, 李洪杰. 小麦抗白粉病基因Pm21的分子鉴定和标记辅助选择[J]. 遗传学报, 1999, 26(6): 673-682.
[12] 薄天岳, 叶华智, 李晓兵. 亚麻抗枯萎病基因FuJ7(t)的分子标记[J]. 中国农业科学, 2003, 36(3): 287-291.
[13] 李凤梅. 黑龙江省南瓜病毒病毒原鉴定和品种资源抗性筛选的研究[D]. 哈尔滨: 东北农业大学. 2000.
[14] 陈洁云. 葫芦科植物病毒病研究[D]. 杭州: 浙江大学, 2003.
[15] 蔡健和. 中国南瓜曲叶双生病毒的生物学、血清学和分子杂交研究[J]. 中国病毒学报, 1994, 9(3): 222-224.
[16] 古勤生. 葫芦科作物主要病毒病害防治研究进展[J]. 中国西瓜甜瓜, 2002(4): 27-30.
[17] 康东木, 许勇. 葫芦科作物抗主要病毒病研究进展[J]. 北京农业科学, 2001(4): 15-20.
[18] Provvidenti. R. 用生物技术和自然抗性获得对葫芦科蔬菜作物病毒病的抗性[J]. 中国蔬菜, 1997(4): 55-57.
[19] Robison R W, Provvidenti R. Test for seedbome transmission of zucchini yellow mosaic virus[J]. Hortscience, 1993, 28 (7): 694-696.
[20] Provvidenti R. Inheritance of resistance to the Florida Strain of Zucchini yellow mosaic virus in watermelon[J]. Hortscience, 1991, 26(4): 407-408.
[21] Provvident R. Sources of resistance of toleance to viruses in accessions of cucurbita maxima[J]. Cucurbit Genet Coop Rep, 1982, 5: 46-47.
[22] Provvident R. Viral Diseases of cucurbits and sourses of resistance[J]. Review of Plant Pathology, 1987, 66(9): 425.
[23] Provvident R. Inheritance of resistance to the florida strain of zucchini yellow mosiaic virus in watermelon[J]. Hortscience, 1991, 26(4): 407-408.
[24] Provvident R. Resistance to viral disease of cucurbits. In: Resistance to viral disease of vegetables: genetics and breeding Oregon[M]. Timber Press, 1993: 8-43.
[25] Provvident R. New American summer squash cultivars possessing a high lever of resistance to a strain of ZYMV from China[J]. Cucurbit Genet Coop Rep, 1997, 20: 57-58.
[26] Provvident R. A source of a high level of tolerance to squash mosaic virus in a melon from China[J]. Cucurbit genet Coop Rep, 1998, 21: 29-30.
[27] Provvident R, Robinson, R W Munger, H M Resistance in feral spieces to six viruses infecting cucurbita[J]. Plant Dis Rep, 1978, 62(4): 326-329.
[28] Provvident R, Robinson, R W Munger, H M Multiple virus resistance in cucurbita species[J]. Cucurbit Genent Coop Rep, 1978, 1: 26-27.
[29] 毕玉平, 单蕾, 王兴军, 等. 双抗TMV+CMV辣椒转基因工程植株的再生及抗病毒鉴定[J]. 华北农学报, 1999, 14(3): 103-108.
[30] 李淑菊, 王惠哲, 霍振荣, 等. 利用RT-PCR对黄瓜病毒病毒原种类进行检测[J]. 华北农学报, 2004, 19(3): 100-102.
[31] 左开井, 孙济中, 张金发. 棉花RAPD分析条件优化探讨[J]. 棉花学报, 1997, 9(6): 304-307.
[32] 沈法富, 于元杰, 尹承佾. 小麦双引物RAPD分析方法的研究[J]. 西北植物学报, 1998, 18(3): 428-432.
[33] 萨姆布鲁克 J, 弗里奇 E F, 曼尼阿蒂斯 T. 分子克隆实验指南[M]. 第2版. 北京: 科学出版社, 1998: 680.

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