Effects of Ultrasonication Treatment on Genetic Transformation by Pollen-Mediated in Apple

doi:10.3321/j.issn:1000-7091.2005.02.005

Abstract

Abstract: The article discussed the effects of additional ultrasonication treatment on the germination of apple(Malus pumila Mill) pollen during pollen-mediated genetic transformation of apple cultivars Danxia and Gala. In order to find out suitable buffer solution and ultrasonication parameters, different pH values, cane sugar concentrations of buffer and various powers, treatment times, treatment number of ultrasonication were set up and examined through experiment. The results showed that the suitable buffer pH value fit for apple pollen treatment was 5.4 and the suitable concentration of cane sugar 10%. It was also indicated that such parameters as the power, time and treatment number of ultrasonication had significant effect on the broken rate and the germinated rate of apple pollen.

Key words: Apple, Pollen, Genetic transformation, Ultrasonication

CLC Number:

S661.101

CAO Qiu-fen, YUE Xin-li, MENG Yu-ping, JI Lan, FAN Xin-ping. Effects of Ultrasonication Treatment on Genetic Transformation by Pollen-Mediated in Apple[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2005, 20(2): 16-18. doi: 10.3321/j.issn:1000-7091.2005.02.005.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.3321/j.issn:1000-7091.2005.02.005

http://www.hbnxb.net/EN/Y2005/V20/I2/16

[1]	LI Haodong, QIN Mengfan, ZHANG Miao, WANG Rongrong, XU Yu, SONG Jia, ZHU Yunlin, HUANG Zhen, XU Aixia. *Effects of Leaf Physiology and Pistil/Stamen Development of Brassica napus* L.to Low Temperature During the Flowering Stage** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 78-86.
[2]	JIA Chaoshuang, WANG Zhihua, WANG Wenhui. Effects of Different Shelf Temperature Combined with 1-MCP Treatment on Texture Traits of Huahong and Huayue Apples [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 128-140.
[3]	ZHANG Zhiwei, FAN Junchen, KANG Liru, JIA Ruifang, TIAN Zaimin, ZHAO Jun. *Effect of StRab5b* Gene on Anthocyanin Synthesis in Potato** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 28-33.
[4]	LU Xiaoyue, WANG Ziye, HAN Jianwei, XU Lu, ZHANG Xiaofei, HAN Jie, WANG Zhigang, LIU Yue, SUO Xiangmin, YAN Aihua. Effects of Pyroligneous Acids on the Growth and Main Characteristics of Replanted Soil of Malus angustifolia [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(3): 175-185.
[5]	TANG Long, ZHAO Yuwei. *Over-expression of PnSMO1.1* Gene Improves Salinity-stress Resistance of Transgenic Pharbitis nil Seedlings** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(2): 1-8.
[6]	ZHAO Huiying, YANG Guangkai, GAO Yan, ZHANG Xiaojun, HAO Yanyan. *Cloning of Gene MdSGR2* from Apple and Construction of Plant Overexpression Vector** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(2): 42-48.
[7]	WU Honghong, DUAN Xuefen, GUO Yangdong, ZHANG Xichun. *Effects of Transcription Factor SlMYB-related 2* on Cold Tolerance of Tomato** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 35-41.
[8]	LI Liang, YU Zhuo, YU Xiaoxia, YANG Dongsheng, LU Qianqian, LI Jingwei, LI Jiaqi, LIU Yufei. Analysis of Cytology and SSR for 6 New Potato Strains [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(2): 74-80.
[9]	WANG Qian, CAI Huacheng, WANG Shuting, ZHANG Xiaojun, GAO Jingdong, DU Xuemei, LI Chunyan, HAO Yanyan, YANG Tingzhen. Comparision on Growth and Photosynthetic Characteristics of Fuji Apple with Two Intermediate Rootstocks under High Spindle [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(1): 108-115.
[10]	FU Jing, SUN Meng, MA Chunhui, HUANG Yonghong. Metabolomic Analysis of Four Allium Species and Their Inhibitory Effects on Botryosphaeria berengriana [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 363-370.
[11]	DING Chaojie, GUAN Yuanyuan, LI Chengwei. Cloning,Genetic Transformation and Tissue Expression Pattern Analysis of Wheat miR395a Precursor [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(4): 52-56.
[12]	HE Fumeng, LI Xiuyu, ZHAO Xiaocan, WU Jiawen, ZHU Yuanfang, ZHOU Lei, SHI Qihai, LIU Di, LI Fenglan. *Analysis of Disease Resistance and Physiological Characteristics of Transgenic Tobacco with Solanum tuberosum StPR1* Gene** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(3): 184-192.
[13]	LI Lun, KE Jie, HUANG Gang, LIU Hongshi, SONG Yanni, ZHANG Yingjie, CHEN Lihong. *Enhanced Oxidative Stress Tolerance of Transgenic Tobacco Plants by Overexpression of Brachypodium distachyon BdDREB1-like* Gene** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(2): 1-8.
[14]	LI Shijun, YIN Baoying, LI Jia, LI Zhongyong, ZHANG Xueying, XU Jizhong. Changes of Ethylene, Related Enzymes and Their Genes Expression During Softening of Premature Apple Fruits [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(6): 104-109.
[15]	LIU Dong, GUO Na, MA Jianfu, LI Airong. Exogenous DNA of Wild Flax Introduced into Cultivated Flax and Its RAPD Molecular Verification of Transferred Progenies [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(S1): 29-32.

Please choose a citation manager

Content to export