Study on the Major Factors Affecting Transformation Efficiency by Agrobacterium-mediated in Brassica napus

doi:10.3321/j.issn:1000-7091.2005.02.004

Abstract

Abstract: The hypocotyls segments were transformated by Agrobacterium mediated transformation in Brassica napus. Some factors affect shoot regeneration of transformated cells were studied. Our study revealed that, for Agrobacterium -mediated transformation, the hypocotyls segments were preconditioned on preconditioning medium for 3 days prior to infect with A.tumefaciens and the addition of 30 μmol/L AgNO₃ in the regeneration medium were prerequiste for obtained high transformation efficiency of explants. Explants were submerged in the A.tumefaciens suspension at the concentration of OD₆₀₀=0.2-0.4 for 5 minutes and placed on the co-cultivation medium pH 5.2 could result in high transformation efficiency of explants.

Key words: Brassica napus L., Transformation, Agrobacterium-mediated transformation

CLC Number:

S565.03.53

WANG Jing-xue, DU Jian-zhong, RONG Er-hua, LI Xiao-can, SUN Yi. Study on the Major Factors Affecting Transformation Efficiency by Agrobacterium-mediated in Brassica napus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2005, 20(2): 12-15. doi: 10.3321/j.issn:1000-7091.2005.02.004.

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