A Study of Agrobacterium-mediated Bar Gene Trans formation on Wheat

doi:10.3321/j.issn:1000-7091.2003.01.004

Abstract

Abstract: A bar gene was introduced into wheat immature embryos(10 days af ter pollination) by Agrobacte rium tume faciens mediated trans formation. PPT(5‰) selec t ion and further PCR and PCR-Southern blot test demons trated that bar gene has been integrated into plant genome. The major procedures for obtaining high frequency re generated plants were also discus sed.

Key words: Wheat, Embryo, Agrobacte rium tume faciens, Bar gene, Trans formation

CLC Number:

S512.1

LIANG Xue lian, SUN Yi, GUO Ping yi, LIU Hui min, LIU Shao xiang, WANG Jing xue, XIE Zhi hong. A Study of Agrobacterium-mediated Bar Gene Trans formation on Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2003, 18(1): 12-16. doi: 10.3321/j.issn:1000-7091.2003.01.004.

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