RAPD Analysis and Identification of Chilling Resis tance of Cellular Variation in Eggplant

doi:10.3321/j.issn:1000-7091.2003.01.005

Abstract

Abstract: Chilling-res istant plants regenerated from anther of eggplant s train E-9903 under low temperature s tres scul ture were used as experimental material. DNA was ext racted fr om young leaves using SDS method. Forty 10-base-long oligonucleotide primers were used in polymerase chain reaction. The index of chil ling injury of cellular variation S1 progeny were measured. Results sho wed that polymorphism existed when primer OPK12 was used in polymerase chain reaction.The index of chilling injury of c ellular variation S1 progeny were much lower than that of contr ol strain.

Key words: Eggplant, RAPD(Random Amplified Polymorphic DNA), Chilling resistance

CLC Number:

S641.1

ZHAO Fu kuan, GAO Xia hong, CHENG Ji hong, FAN Shuang xi, YU Ying. RAPD Analysis and Identification of Chilling Resis tance of Cellular Variation in Eggplant[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2003, 18(1): 17-20. doi: 10.3321/j.issn:1000-7091.2003.01.005.

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