Effects of Brassinolide on Seed Germination and Seedling Physiology of Maize under Low Temperature Stress

doi:10.7668/hbnxb.201751362

Abstract

Abstract: In order to explore the effects of brassinolide(BR) on seed germination and seedling physiological characteristics of maize under low temperature stress, the maize hybrids Tiannong 9 and Tianhe 1 were used as the materials to study the changes of agronomic traits and physiological properties after their seeds were soaked or sprayed with BR. The results showed that exogenous BR treatment could effectively alleviate the inhibition of maize growth under low temperature stress. The best treating concentration was 0.1 mg/L BR, which increased the germination rate, the biomass of overground and underground parts, the SPAD value, the activities of SOD, POD and CAT, and the contents of proline, soluble sugar and soluble protein, while decreased the MDA content and the relative conductivity in maize seedlings. Compared with the low temperature control, the maize hybrids Tiannong 9 and Tianhe 1 treated with exogenous BR of 0.1 mg/L initial germination rate increased by 18.89 and 11.10 percentage points respectively, the final germination rate increased by 6.67 and 18.89 percentage points, their aboveground dry weight increased by 55.92% and 49.15%, their underground dry weight raised by 38.79% and 14.26%, their relative electrical conductivity decreased by 7.33% and 6.80%, their CAT activity increased by 110.58% and 120.43%, and their proline content increased by 66.59% and 54.63%, respectively. It suggested that the relieving effects of 0.1 mg/L BR treatment on the two maize varieties under low temperature stress were different, Tiannong 9 being better than Tianhe 1. The maize varieties with different cold resistances had different adaptability to different BR concentrations.

Key words: Maize, Chilling resistance, Brassinolide, Germinate, Physiological property

CLC Number:

S513.01

SUN Yujun, WU Yue, MA Dezhi, LÜ Jinying, HE Yunhan, GONG Lei, LIU Zhe, GAO Lidan, LI Na, YAN Dong, ZHU Jinghuan, YANG Deguang. Effects of Brassinolide on Seed Germination and Seedling Physiology of Maize under Low Temperature Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(3): 119-128. doi: 10.7668/hbnxb.201751362.

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