Selection of SSR Core Primers and Varietals Purity Identification in Eggplant

doi:10.3969/j.issn.1000-7091.2012.04.018

Abstract

Abstract: To establish a fast and accurate hybrid identification method using molecular markers urgently. 237 pairs of SSR markers were used for polymorphic markers selection in three eggplant varieties,and purity identifica- tion was conducted in both laboratory and field in order to get finger printing information. The results showed that ultimately 15 pairs of polymorphic primers used in purity identification was selected. Application of these primers, variety 1-2010,3-2010 and 9-2010 purity results were 98%,96% and 100%, finger printing information was a- chieved on this basis. Field test results consistent with laboratory ones, it identified purity of eggplant hybrids using molecular markers was feasible and efficient,which has a certain theoretical and practical values.

Key words: Eggplant, Purity identification, Polymorphic primers SSR

CLC Number:

S641.1

WANG Li-ying, QIAO Jun, SHI Yao, WANG Zhen-ying, LI Su-wen. Selection of SSR Core Primers and Varietals Purity Identification in Eggplant[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(4): 98-101. doi: 10.3969/j.issn.1000-7091.2012.04.018.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.3969/j.issn.1000-7091.2012.04.018

http://www.hbnxb.net/EN/Y2012/V27/I4/98

References

[1] 《小麦指纹图谱数据库的建立及SSR分子标记试剂盒的研发》[J].作物学报,2006(12).

[2] 《RAPD技术在茄子种子鉴定中的应用研究》[J].黑龙江八一农垦大学学报,2003(2).

[3] 《利用微卫星标记进行凉山半细毛羊亲权鉴定的研究》[J].遗传,2005(1).

[4] 《中国主栽水稻品种微卫星标记数据库的初步构建》[J].中国水稻科学,2006(5).

[5] 《玉米品种DNA指纹数据库构建的标准化规范》[J].分子植物育种,2007(1).

[6] 《应用SSR标记技术鉴定玉米杂交种的纯度》[J].山西农业科学,2007(1).

[7] 《建立小麦品种DNA指纹的方法研究》[J].作物学报,2007(10).

[8] Roder M S, Wendehake K, Korzun V, et al. Construction and analysis of a microsatellite-based database of Europe- an wheat varieties [J]. Theoretical and Applied Genetics, 2002,106:67 - 73.

[9] Bredemeijer G M M, Cook R J, Ganal M W,et al. Con- struction and testing of microsatellite database containing more than 500 tomato varieties [J]. Theoretical and Ap- plied Genetics,2002,105 : 1019 - 1026.

[10] 刘朝.茄子、辣椒种子纯度RAPD检测技术体系研究[D].泰安:山东农业大学,2002.

[11] Nunome T, Negoro S, Kono I,et al. Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L. ) [J]. Theoretical and Applied Genetics,2009,119 : 1143 - 1153.

[12] 《茄子种质资源形态性状的多样性分析》[J].安徽农学通报,2007(18).

[13] 《茄子栽培种与野生种质资源遗传关系的RAPD分析》[J].上海交通大学学报：农业科学版,2008(2).

[14] 《SSR分子标记方法鉴定杂交向日葵品种纯度研究》[J].山西农业科学,2008(5).

[15] 《上海地区植物新品种DUS测试技术体系的初步构建》[J].上海农业学报,2009(1).

[16] 《中国88个马铃薯审定品种SSR指纹图谱构建与遗传多样性分析》[J].作物学报,2009(8).

[17] 《RAPD分析用的梨DNA提取方法》[J].遗传,1998(4).

[18] 《DNA分子标记技术及其在药用植物研究上的应用前景》[J].生物工程进展,1998(4).

[19] 《利用SSR分子标记建立辣椒纯度鉴定体系》[J].辣椒杂志,2010(1).

[20] 《利用SSR技术对黄瓜新品种津优35号进行种子纯度鉴定》[J].北方园艺,2010(10).

[21] 《中国不同省份籼稻地方品种的指纹图谱分析》[J].中国农业科学,2010(11).

[22] 《中国棉花主栽品种DNA指纹图谱构建及SSR标记遗传多样性分析》[J].中国农业科学,2011(1).

[23] 《我国棉花新品种权保护现状浅析》[J].中国棉花,2010(12).

[24] 《基于SSR优化反应体系的茄子亲子鉴定》[J].山西农业科学,2011(8).

[25] 《利用SSR进行品种纯度鉴定的研究进展》[J].天津农业科学,2011(4).

[26] 《品种纯度和真伪的DNA分子标记鉴定及其应用》[J].农业生物技术学报,2000(2).

[27] 《关于植物随机引物扩增多态性DNA标记的可靠性问题》[J].植物生理学通讯,2000(1).

[28] 《东北虎微卫星DNA遗传标记的筛选及在亲子鉴定中的应用》[J].动物学报,2003(1).

[29] 《番茄分子连锁图谱的发展和分子标记辅助育种》[J].天津农业科学,2004(1).

Please choose a citation manager

Content to export