SSR Molecular Markers Identification of Pepper Hybrid and Comparative Analysis with Phenotypes

doi:10.7668/hbnxb.2014.01.013

Abstract

Abstract: Establishing a rapid and stable seed purity identification technology system is the effective measure for controlling the pepper hybrid quality. The development of molecular marker techniques could provide an accurate and fast way for identifying the crops hybrid purity. In this study,SSR markers were applied to testing the purity of Rela No. 3. 10 pairs among 85 pairs of SSR primers showed significant polymorphisms between the parents of Rela No. 3. All of them were co-dominant markers. In order to improve the accuracy of identification results,three codominant SSR markers located on different chromosomes were selected for purity test of Rela No. 3. The seed purity was 99. 49%. Comparative analysis showed that the identification results of molecular markers and phenotypes were highly consistent. The study results indicated that SSR markers could be used for hybrid purity test of Rela No. 3, quickly and accurately.

Key words: Pepper, Hybrid, SSR markers, Purity identification

CLC Number:

LIU Zi-ji, LI Jing-ting, YANG Yan, CAO Zhen-mu. SSR Molecular Markers Identification of Pepper Hybrid and Comparative Analysis with Phenotypes[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(1): 69-72. doi: 10.7668/hbnxb.2014.01.013.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2014.01.013

http://www.hbnxb.net/EN/Y2014/V29/I1/69

References

1 盖树鹏，盖伟玲，王日新6个玉米杂交种种子纯度的SSR鉴定[J]种子，2010,29(7);44-47
2 兰刚，董军刚，孟倩，等油菜杂交种合油杂2号纯度鉴定的SSR引物筛选[J]西北农业学报，2012,21(9);74-78
3 Salgado K C P C, Vieira M G G C, Von Pinho E V R. Ge-netic purity certificate in seeds of hybrid maize using mo-lecular markers[J]Revista Brasileira de Sementes,2006,28(1):169-175
4 李智军，曾晶，龙卫平，等黄皮尖椒秀黄F₁种子纯度的RAPD鉴定[J].广东农业科学，2012(23);136 -138
5 李成伟，李卓杰，陈润政用RAPD方法鉴定杂交辣椒种子纯度[J]种子，1999(2);5-6
6 王得兀，王永飞粤椒1号辣椒种子纯度的RAPD检测研究[J]中国辣椒，2002 (1);19-22
7 刘子记，曹振木，杨衍应用分子标记技术检测作物杂交种纯度研究进展[J]种子，2013,32(6);48-51
8 管志坤，罗双霞，李艳霞，等利用SSR标记鉴定油绿3号大自菜品种纯度[J]种子，2011,30(9):34-39
9 杨瑞环，崔兴华，李鹏宇，等SSR技术检测‘津优48' 种子纯度试验[J]北方园艺，2012(16);108-109
10 鲁忠富，徐沛，吴晓花，等SSR分子标记技术在瓤瓜种子纯度快速鉴定中的应用[J]浙江农业学报，2012,24(4);578-581
11 王利英，乔军，石瑶，等茄子SSR多态性引物的筛选及品种纯度鉴定[J]华北农学报，2012,27(4): 98-101
12 赵振卿，盛小光，虞慧芳，等花椰菜新品种浙801杂交种纯度的SSR鉴定[J]长江蔬菜，2011(18);18-20
13 Liu Z J,Zhu J,Cui Y,et aL.Identification and compara-five mapping of a powdery mildew resistance gene de-rived from wild emmer(Triticum turgidum var. dicoc-cone chromosome 2BS[J]Theor Appl Genet, 2012，124(6):1041-1049
14 Smith 0 S,Smith J S C,Bowen S L,et al. Similarities a-mong a group of elite maize inhreds as measured by ped-igree, Fi grain yield, grain yield, heterosis，and RFLPs [J]. Theor Appl Genet,1990, 80 (6); 833-840
15 葛菊芬，张鲁刚，程智慧，等利用RAPD技术快速鉴定辣椒杂交种纯度的研究[J]辣椒杂志，2010(4); 29-32
16 王玲平，戴丹丽，吴晓花，等AFLP分子标记技术在浙蒲2号种子纯度快速鉴定中的应用[J]浙江农业学报，2008,20(2);84-87
17 田筑萍，唐容，吴有祥，等利用SSR指纹图谱技术对杂交油菜种质鉴定的研究[J]种子，2008,27(6); 69-71
18 武岩军，许晶，车星星应用SSR标记技术鉴定强盛16号玉米单交种纯度[J]山西农业科学，2012, 40(6);599-602
19 张庶，周新成，李利斌，等利用EST-SSR标记鉴定大自菜杂交种纯度的研究[J]天津农业科学,2010，16(6):1-4
20 乔军，王利英，石瑶利用SSR进行品种纯度鉴定的研究进展[J]天津农业科学，2011,17(4);45-51

[1]	LING Chen, ZHAO Hong, YAN Jun, MA Yingxue, FENG Yanfang, ZHANG Jingli, LI Bo, DENG Chao, XU Zhenjiang. Establishment and Application of Variety Identification System Based on SSR Markers for Lettuce [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 34-44.
[2]	JIA Zhiqiang, XU Yunyu, GAO Xue, TAO Hongzheng, CHEN Zengmin, LIU Yating, LI Yongzhong. *Cloning,Subcellular Positioning of Pepper CaWRKY30* Transcription Factor, and Its Expression Analysis by Tomato spotted wilt orthotospovirus Infected** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(3): 186-192.
[3]	GUO Fangrui, LIU Yi, YANG Kexin, CHU Meirong, ZHANG Quanfa, ZHANG Yafei, WANG Shutong, CAO Keqiang. *Screening of Fusarium oxysporum* T-DNA Inserted Mutants and Analysis of Inserting Genes** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(3): 206-212.
[4]	SHI Ailong, WANG Lihuang, LI Wenxiu, WANG Xuehua. Effects of Water and Fertilizer on Agronomic Characters and Yield of Double-cropping Hybrid Rice [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(3): 145-157.
[5]	LEI Yang, QIAO Ning, BAI Yang, YANG Yuhua. Effects of Epibrassinolide on Physiological Characteristics and Resistance Genes of Pepper Seedlings under Severe Cadmium Stress [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 99-106.
[6]	CHEN Siyu, YANG Xue, YANG Lingling, YAN Zhaoling, HAN Xiaoyu, LI Qinglun, LI Honglian, CHEN Linlin, SUN Bingjian, SHI Yan. Screening of the Host Factors Interacting with P22 Protein of Cucurbit chlorotic yellows virus [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(4): 197-204.
[7]	LUO Jian, XU Chunmiao, ZHANG Guobin, YU Jihua. Bioinformation Analysis and Expression Analysis of NHX Genes Family in Pepper [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(3): 15-24.
[8]	CUI Baowei, LIU Quanyong, QIN Guangli, ZHU Junpeng. Effect of Combined Application of Fermented Sheep Manure and Soil Loosening and Root Promoting Agent on Soil Physical and Chemical Properties Yield and Quality of Red Cluster Pepper [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(2): 182-187.
[9]	YI Liuxi, SA Rula, WANG Shuyan, LI Zhiwei, SIQIN Bateer, LI Qiang. SSR Correlation Analysis of Linseed Lignan Content [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(1): 63-70.
[10]	CHAI Ali, CHEN Lida, XU Shuai, PATIGULI Aisimutuola, WANG Lili, SHI Yanxia, XIE Xuewen, LI Lei, LI Baoju. *Molecular Identification of Co-infection of Pepper mild mottle virus* and Cucumber mosaic virus on Pepper in Kashgar, Xinjiang** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 332-337.
[11]	MENG Yaning, YAN Libin, ZHANG Hongxiao, ZHAO Haitao, FAN Yanqin. *Screening and Detection of Resistance to Phytophthora* Blight Gene in Pepper Using Molecular Markers** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 338-341.
[12]	HE Dan, ZHANG Jiaorui, HE Songlin, LIU Hongli, WANG Zheng, LIU Yiping. Cloning and Expression Analysis of Distant Hybridization Incompatibility PlABCF3 Gene from Paeonia [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(6): 81-89.
[13]	LUO Jian, ZHANG Guobin, CHE Xusheng, QIN Qijie. Bioinformation Analysis and Expression Analysis of GME Genes Family in Ascorbic Acid Biosynthesis of Pepper [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(4): 71-78.
[14]	DING Weihua, JIANG Xiaoling, FENG Suwei, JIN Liqiao, LU Yuping, RU Zhengang. Effects of Sowing Rate on Population Photosynthetic Characteristics, Material Accumulation and Yield of BNS-type Hybrid Wheat [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(4): 113-119.
[15]	GUO Zhaolai, SUN Xudong, YANG Yongping, XU Huini. Screening and Identifying of Interaction Protein AtLBD15 in Arabidopsis thaliana [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(5): 52-58.

Please choose a citation manager

Content to export