Genetic Transformation of Diploid Potato and Expression Suppression of Its Protein Kinase Gene StPki

doi:10.7668/hbnxb.2014.01.014

Abstract

Abstract: Protein kinase gene StPki was cloned from bacterial wilt-resistant diploid potato ED13,and its RNAi plant expression vector was constructed. The expression vector pCHF1-StPki was introduced into Agrobacterium strain LBA4404 and the recombinant strain LBA4404(pCHF1-StPki) was used to transform ED13 stem segment. In the presence of CaMV35S promoter-specific primers,Gen-resistant regeneration plants were detected by PCR and transgenic potato plant producing 500 bp amplification product were obtained. Semi-quantitative RT-PCR analysis indicated that the transcription of StPki gene had beed significantly restrained in the transgenic ED13 plant.

Key words: Potato, Diploid, Protein kinase gene, Genetic transformation, Expression suppression

CLC Number:

YANG Yu, GUO Xiao, GUO Bao-tai, YANG Xiao-hui, SHAN Wei-wei, JIN Li-ping, MA Wei-qing, LI Guang-cun. Genetic Transformation of Diploid Potato and Expression Suppression of Its Protein Kinase Gene StPki[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(1): 73-77. doi: 10.7668/hbnxb.2014.01.014.

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