Cloning and Sequence Analysis of the Gene for 28 kD Protein from Luotian Isolate of Wheat Yellow Mosaic Virus

doi:10.3321/j.issn:1000-7091.2003.01.003

Abstract

Abstract: A cDNA clone (pUW28-10) containing 28 kD protein gene from Luot ian isolate of wheat yellow mosaicvi rus(WYMV) was obtained by RT-PCR. Sequence analysis revealed that nucleot ides sequence of 28 kD protein gene varied among dif ferent isolates of wheat yellow mosaic vi rus. 28 kD protein genes of the Luotian and Huangchuan isolates comprised 762 nucleot ides(nt) and were less 3 nt than others, located at the si te of 326, 327 and 336 nt, respectively. The di fferent isolates shared homologies ranging from 92. 1% to 96. 9% in nucleotide sequence and 89. 8% to 97. 3% in amino acid (aa) sequence.

Key words: Wheat yellow mosaic virus, 28kD protein gene, cDNA cloning, Sequence analysis

CLC Number:

S435.121

DONG Jia hong, YU Jia lin, HAN Cheng gui, FANG Shou guo, LIU Yi. Cloning and Sequence Analysis of the Gene for 28 kD Protein from Luotian Isolate of Wheat Yellow Mosaic Virus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2003, 18(1): 9-11. doi: 10.3321/j.issn:1000-7091.2003.01.003.

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