Abstract: Microspore derived embryos of Chinese cabbage (Brassica campestris ssp.pekinensis)were bruised with crushed glass while coculturing with Agrobacterium,strain C58 The strain harbours vector pDHB321 with CaMV 35S driving bar gene that confers resistance to Basta,a phophinothricin (PPT) based herbicide.There are six green plants regenerated on the selective medium with 5 mg/L PPT out of nearly one thousand treated embryos.Having sprayed a 1% solution of the commercial preparation Basta on one leaf of each plant,we found that all of the treated leaves withered then died.When doing the leaf disc assay on MS medium containing 2 mg/L BA,1 mg/L NAA,10 mg/L PPT,it was found that one of these plants remained clear green,while the control turned completely yellow The former was selfed and its seeds were collected Four of its progenies showed distinctly resistance to the spraying of 1% Basta Results of Southern blottings realved that the bar gene had been integrated into the genome of the plants Microspores of these transformants were cultured further,and plants induced The Basta resistance segregation ratio showed that the transformants were heterogametic.

Key words: Brassica campestris ssp pekinensis, Microspore derived embryo, Agrobacterium tumefaciens, Bar gene, Herbicide resistance, Southern blot

CLC Number: 

• S634.103