Abstract: Resveratrol is a kind of phytoalexins which carl defence pathogen infection when suffered stresses.And resveratrol synthase,a kind of rate-limiting enzymes in Res synthesis pathway,plays key role during Res synthesis procedure.Rice blast,which caused by Magnaporthe grisea,is an extremely hazardous and widely distributed fun-gus disease.In this study,eDNA of resveratrol synthase gene was isolated using peanut total RNA as template by RTPCR amplification.The plant expression binary vector of RS was then constructed using modified pCAMBIAl300 as framework plasmid which using maize ubiquitin promoter and biosecurity screening marker bar instead of 35S promoter and Hygromycin by genetic engineering.The mature embyro of rice Shengdao 13 were transformed by the recombi· nated vector through agrobacterium-mediated transformation,and the positive transformants were further screened by Basta and PCR analysis.The work above will provided new materials for RS gene function identification,and shed some light for new rice germplasm innovation of disease resistence in the future.

Key words: Resveratrol(Res), Resveratrol synthase gene PNRSC, Rice blast, Ubiquitin promoter(Ubi), bar gene, Basta, Cultivation of new germplasm of rice disease resistance

CLC Number: 

• Q78