Abstract: hlL-12 DNA was cloned into plant expression vector pB Ⅱ21 under the control of 35 S pronroter,and thenpBⅡ21-h1L-12 was transferred into Agrobacterium tumefaciens strain EHA105 through the assistant plasmid pRK2013 bytriparental cross. The recombinant plasmid was transformed into potato stems induced by Agrobacterium tumefaciensEHA105.28 transformed plants in all transformed plants were selected by kanamycin selective medium. The hIL-12 genewas found from 22 transformed plants by PCR. ELISA assay showed that the protein expression of the stems and leaves of 2 transgenic potatoes in 5 transgenic potatoes selected at random in the 22 transformed plants was obviously different fromthat of control getup (p<0. 001),and the content of hlf,-12 in the tc}o transgenic potatoes was (3 714. 0士8. 8)pg/ g and(3 465. 0士185. 0)pg/ g respectively. It was sure that the foreign gene was integrated into the genome and expressed inpotato,resulting in two transgenic plants. It lays an experimental foundation for further research of extraction and purifica-tion of the hIL-12 and bioactivity detection.

Key words: Rotato, Human interleukin 12, Cenetic transformation, Agrobacterium tumefaciens

CLC Number: 

• Q789