Structural and Expression Analysis of GhCAD1 Gene from Gossypium hirsuturm L.

doi:10.7668/hbnxb.2011.02.006

Abstract

Abstract: According to the cDNA sequence from cotton GhCADl gene,primers were designed.PCR method was used to clone GhCADl gene and the expression of GhCADl gene in different tissues of cotton was analyzed by semi-quantitative RT-PCR.A length of 2898 bp sequence from genomic DNA of GhCADl was cloned by PCR.The genomic DNA of GhCADl contains five exonsand four introns.Analysis of these introns indicated that they are rich in AT.Furthermore,a SBF-1 factor binding site was identified in the first intron of GhCADl.Semi-quantitative RT-PCR analysis revealed that GhCADl can be expressed in different kinds of cotton tissues,and its mRNA accumulated most abundantly in leaf.GhCADl expression in cotton tissues was leaf>boll shell>bract>petal>root> cotyledon.

Key words: Gossypium hirsuturm L．, GhCADl, Intron, Expression analysis

CLC Number:

Q789

NI Zhi-yong, LU Shu-ping, LI Bo, FAN Ling. Structural and Expression Analysis of GhCAD1 Gene from Gossypium hirsuturm L.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(2): 26-29. doi: 10.7668/hbnxb.2011.02.006.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2011.02.006

http://www.hbnxb.net/EN/Y2011/V26/I2/26

References

[1] 喻树迅.棉花纤维品质功能基因组学研究与分子改良研究进展[J].中国基础科学,2007,4:18-21.

[2] Shi Y H,Zhu S W,Mao X Z,et al.Transcriptome profiling,molecular biological,and physiological studies reveal a major role for ethylene in cotton fiber cell elongation[J].The Plant Cell,2006,18:651-664.

[3] Yves A G,St phane B,Tony A,et al.Transcript profiling during fiber development identifies pathways in secondary metabolism and cell wall structure that may contribute to cotton fiber quality[J].Plant Cell Physiol,2009,50 (7):1364-1381.

[4] 倪志勇,吕萌,马文静,等.棉花咖啡酰辅酶A-O-甲基转移酶基因的克隆及表达[J].西北植物学报,2009,29(10):1946-1953.

[5] 倪志勇,李波,吕萌,等.棉花肉桂醇脱氢酶基因的克隆与分析[J].华北农学报,2010,25(2):23-29.

[6] 倪志勇,马文静,吕萌,等.棉花肉桂醇脱氢酶基因GhCAD6的克隆及正义、反义与RNAi干扰载体的构建[J].华北农学报,2009,24(6):21-26.

[7] 倪志勇,王娟,吕萌,等.棉花4-香豆酸辅酶A连接酶基因克隆及原核表达[J].西北植物学报,2010,30(3):429-436.

[8] 倪志勇,吕萌,李波,等.棉花咖啡酸-O-甲基转移酶基因的克隆及特征分析[J].中国农业科学,2010,43(6):1117-1126.

[9] Whetten R,Sederoff R.Lignin biosynthesis[J].The Plant Ce11,1995,7(7):1001-1013.

[10] Fan L,Shi W J,Hu W R,et al.Molecular and biochemical evidence for phenylpropanoid synthesis and presence of wall-linked phenolics in cotton fibers[J].Journal of Integrative Plant Biology,2009,51 (7):626-637.

[11] Xu Z S,Ni Z Y,Li Z Y,et al.Isolation and functionalcharacterization of HvDREBl-a gene encoding a dehydration responsive element binding protein in Hordeum vulgare[J].J Plant Res,2008,122 (1):121-130.

[12] 倪志勇,徐兆师,刘丽,等.小麦转录因子TaDREB6基因的克隆及鉴定[J].麦类作物学报,2008,28(3):357-363.

[13] Raes J,Rohde A,Christensen J H,et al.Genome-wide characterization of the lignification toolbox in Arabidopsis[J].Plant Physio1,2003,133 (3):1051-1071.

[14] Le H H,Nott A,Moore M J.How introns influence and enhance eukaryotic gene expression[J].Trends in Biochemical Sciences,2003,28 (4):215-220.

[15] Wang S,Wang J W,Yu N,et al.Control of plant trichome development by a cotton fiber MYB gene[J].The Plant Cell,2004,16 (9):2323-2334.

[16] Lawton m A,Dean S M,Dron M,et al.Silencer region of a chalcone synthase promoter contains multiple binding sites for a factor,SBF-1,closely related to GT-1[J].Plant Mol Biol,1991,16 (2):235-249.

[17] Maniatis T,Reed R.An extensive network of coupling among gene expression machines[J].Nature,2002,416(4):499-506.

[18] Jeon J S,Lee S,Jung K H,et al.Tissue-preferential expression of a rice a-tubulin gene,OsTubA1,mediated by the first intron[J].Plant Physiology,2000,123 (3):1005-1014.

[19] Curi G C,Chan R L,Gonzalez D H.The leader intron of Arabidopsis thaliana genes encoding cytochrome c oxidase subunit 5c promotes high-level expression by increasing transcript abundance and translation efficiency[J].Journal of Experimental Botany,2005,56 (419):2563-2571.

[20] Ruao M B,Liao W B,Zhang X CH,et al.Analysis of the cotton sucrose synthase 3 (Sus3)promoter and first intron in transgenic Arabidopsis[J].Plant Science,2009,176(3):342 -351.

Please choose a citation manager

Content to export