Protein-Protein Interaction Between Members of NH1 Family in Bimolecular Fluorescence Complementation Assay

doi:10.7668/hbnxb.2011.02.007

Abstract

Abstract: NPRl(Non-expresser of pathogenesis related genes 1)is the master regulator of salicylic acid-mediated systemic acquired resistance.Over-expression of Arabidopsis NPRI and rice NHl(NPRl homologl)/OsNPRl in rice resuhs in enhanced resistance.There are four rice NPRl-paralogs in the rice genome,they are family members of NHl.Protein-protein interaction between members of NHl family were studied in vivo by bimolecular fluorescence eomplementation assay(BiFC,Or split YFP).NHl,NH3,NH4,and NH5 all can interact with itself to generate fluores-eence signals.They Can also interact with other members in the family.But NH2 has no interaction with anyone.

Key words: Rice, NH1 family members Bimolecular fluorescence complementation assay, Protein-protein interaction

CLC Number:

BAI Wei, SUN Hai-lian, Mawsheng Chern, Randy Ruan, Pam Ronald, FAN Ming-shou. Protein-Protein Interaction Between Members of NH1 Family in Bimolecular Fluorescence Complementation Assay[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(2): 30-34. doi: 10.7668/hbnxb.2011.02.007.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2011.02.007

http://www.hbnxb.net/EN/Y2011/V26/I2/30

References

[1] 刘若晗.植物抗病性分子机制研究进展[J].内蒙古农业科技,2009(6):36-37,85.

[2] 高银.植物抗逆机制与基因工程研究进展[J].内蒙古农业科技,2007(5):75-78.

[3] 崔晓卉,赵艳芳.小麦抗白粉病研究进展及其在育种上的应用[J].内蒙古农业科技,2008(2):97-99.

[4] Uwe Conrath.Systemic acquired resistance[J].Plant Signaling & Behavior,2006,1 (4):179 -184.

[5] A Corina Vlot,Daniel F Klassig.Systemic acquired resistance:the elusive signal(s)[J].Current Opinion in Plant Biology,2008,11:436 -442.

[6] Van Oosten V R.Signal signature and transcriptome changes of Arabidopsis thaliana during pathogen and insect attack[J].Mol Plant-Microbe Interact,2005,18:923 -937.

[7] Grant M,Lamb C.Systemic immunity[J].Curt Opin Plant Bio1,2006,9:414-420.

[8] Glazebrook J.Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens[J].Annu Rev Phytopathol,2005,43:205-227.

[9] 额尔敦,邱淑琴.禾谷类作物基因转移进展[J].内蒙古农业科技,1991(2):38-40,37.

[10] 李恋.生物技术在植物育种上的新应用[J].内蒙古农业科技,2006(3):52-54,56.

[11] Thomma B P H J,Penninckx I A M A.The complexity of diseasesignaling in Arabidopsis[J].Curr Opin Immunol,2001,13:63-68.

[12] Cao H,Bowling S A.Characterization of an Arabidopsis mutant that is nonresponsive to inducers of systemic acquired resistance[J].Plant Cell,1994,6:1583-1592.

[13] Delaney T P,Friedrieh L.Arabidopsis signal transduction mutant defective in chemically and biologically induced disease resistance[J].Proc Natl Acad Sci,1995,92:6602-6606.

[14] Glazebrook J,Rogers E E.Isolation of Arabidopsis mutants with enhanced disease susceptibility by direct screening[J].Genetics,1996,143:973-982.

[15] Ryals J,Weymann K.The Arabidopsis NIM1 protein shows homology to the mammalian transcription factor inhibitor I B[J].Plant Cell,1997,9:425-439.

[16] Shah J,Tsui.Characterization of a salicylic acid-insensitive mutant(sail) of Arabidopsis thaliana,identified in a selective screen utilizing the SA-inducible expression of the tmns2 gene[J].Mol Plant Microbe Interact,1997,10:69-78.

[17] Cao H,Glazebrook J.The Arabidopsis nprl gene that controls systemic acquired resistance encodes a novel protein containing ankyrin repeats[J].Cell,1997,88:57-63.

[18] Kinkema M,Fan W.Nuclear localization of NPR1 is required for activation of PR gene expression[J].Plant Cell,2000,12:2339-2350.

[19] Zhang Y,Fan.Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR-1 gene[J].Proc Natl Acad Sci USA,1999,96:6523 -6528.

[20] Despres C,DeLong C.The Arabidopsis NPR1⁄NIM1 protein enhances the DNA binding activity of subgroup of the TGA family og bZIP transcription factors[J].Plant Cell,2000,12:279-290.

[21] Boyle P,Su E L.The BTB⁄POZ domain of the Arabidopsis disease resistance protein NPR1 interacts with the repression domain of TGA2 to negate its function[J].Plant Cell,2009,21:3700-3713.

[22] Mou Z,Fan W.Inducers of plant systemic acquired resistance regulate NPR1 function through redox changes[J].Cell,2003,113:1-10.

[23] Chern M,Fitzgerald H A.Over-expression of a rice NPR1 homolog leads to constitutive activation of delense response and hypersensitivity to light[J].Mol Plant Microbe Interact,2005,18:511-520.

[24] Bracha-Drori K,Shichrur K.Detection of protein-protein interactions in plants using bimolecular fluorescence complementation[J].Plant J,2004,40:419-427.

[25] Walter M,Chaban C.Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation[J].Plant J,2004,40:428-438.

Please choose a citation manager

Content to export