Abstract:
To detect the intestinal bacteria quickly and accurately, an experimental procedure was set up using the gene chip technology to detect and identify common intestinal bacteria. Target gene was amplified and hybridized with prepared gene chip. The results showed 23 strains of bacteria in pure culture belong to 4 genera were successfully discriminated under comparatively same condition and a series of specific hybridization maps corresponding to each kinds of bacteria were obtained. Salmonella spp, Shigella spp, Proteus spp, Yersinia enterocolitica, were detected and identified by the technology of gene chip.
Key words:
Gene chip,
Intestinal bacteria,
Detection of intestinal bacteria
CLC Number:
XU Xiao-jing, WEN Si-yuan, HAN Yi. Detection and Identification of Intestinal Bacteria by Oligonucleotide Array[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(1): 182-183. doi: 10.3321/j.issn:1000-7091.2007.01.043.