Screening and Identification of Cellulase-producing Strains from Intestinal Canal of Dendrolimu houi and Research on Cellulase Characterization

doi:10.3969/j.issn.1000-7091.2012.z1.051

Abstract

Abstract: Twenty-eight Cellulase-producing strains were isolated from intestinal canal of Dendrolimu houi． Basing on biochemical， physiological characterization and 16S rRNA sequences blasting， these strains were classifiedinto Enterobacter sp．， Bacillus sp．， Klebsiella sp． and Yokenella sp．． Strain DK4 was found as a higher-yield strainfor cellulase in these strains． A phylogenetic tree was constructed with the published 16S rRNA sequences of relativebacteria species． In the phylogenetic tree， the strain DK4 was in the branch with the Bacillus cereus strain NAV-10( GU056811 ． 1 ) ． The highest cellulase activity of DK4 strain was 19． 24 U /mL when grown at 35℃ ， pH 7． 0 for 70 h．

Key words: Intestinal bacteria, Dendrolimu houi, Cellulase, Identification, Enzyme characterization

CLC Number:

S763.3

SUN You-he, ZHOU Kai-yan, XIONG Zhi. Screening and Identification of Cellulase-producing Strains from Intestinal Canal of Dendrolimu houi and Research on Cellulase Characterization[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.3969/j.issn.1000-7091.2012.z1.051.

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