Abstract: UGT is a kind of glycosyltransferase,which performs glycosylation modification on flavonoids,synthesizes isoflavone glycosides,and plays an important role in the synthesis,transport and storage of isoflavones. In order to explore the function of related genes,the full-length CDS sequence of GmUGT73 gene was cloned from Zhongdou 27 by RT-PCR. The GmUGT73 protein sequence and physicochemical properties were analyzed by online software such as ProtParam tool. The results indicated that the coding region of GmUGT73 gene encoded 464 amino acids with a molecular mass of 51.186 5 ku,a theoretical isoelectric point(pI)of 6.27 and a total atomic number of 7 176. The molecular formula was C₂₂₈₁H₃₅₈₀N₆₀₈O₆₈₅S₂₂. The total positive electroreactive residue of GmUGT7 protein was 37,the total number of negatively charged residues was 41,and the instability coefficient of protein was 36.93,indicating that the protein was stable. The protein had a fat solubility index of 86.19 and a GRAVY value of -0.111,indicating that the protein should be a hydrophilic protein. The linear plant expression vector was ligated to the target fragment,and the pCambia3300-GmUGT73 plant expression vector was successfully constructed. Transformation of soybean hairy roots by Agrobacterium rhizogenes-mediated transformation and obtained transgenic positive roots.The isoflavone content of roots were determined by high performance liquid chromatography. The results showed that the content of isoflavones in the positive roots of transgenic plants was extremely significantly higher than that of the control(P<0.01),indicating that the gene may have the function of promoting the synthesis of soy isoflavones.

Key words: Soybean, Isoflavone, Glycosyltransferase, Candidate gene, Agroinfiltration, Cloning, Functional identification of gene

CLC Number: 

• Q78

• S565.03