The Establishment of Gene Chip for Chicken Infectious Anemia Virus, Reticuloeotheliosis Virus and Avian Leucosis Virus

doi:10.7668/hbnxb.2015.06.014

Abstract

Abstract: To develop a gene chip assay for the detection of Chicken infectious anemia virus,Reticuloeotheliosis virus,Avian leucosis virus subgroups A,C and D based on the specific gene fragment amplified by multiplex PCR.The references sequence of Chicken infectious anemia virus,Reticuloeotheliosis virus and Avian leucosis virus subgroups A,C and D were obtained from GenBank;which were used to design the specific primers and probes.The downstream primers were labeled with Cy3,and gene chip was subsequently prepared.The total DNA was extracted from the three viruses.The product amplified by using PCR with the labeled primers was hybridized with the gene chip.The hybridization signal was detected and analyzed by a fluorescence scanner.The results showed that:the chip was able to simultaneously detect Chicken infectious anemia virus,Reticuloeotheliosis virus and Avian leucosis virus subgroups A,C and D.This method with high sensitivity,which could reach to 10⁶ copies/mL.Furthermore,there was no cross reactions with NDV,MDV and IBDV.This study might provide potential method for rapid surveillance and differential diagnosis of Chicken infectious anemia virus,Reticuloeotheliosis virus and Avian leucosis virus subgroups A,C and D.

Key words: CIAV, REV, ALV, Gene chip

CLC Number:

S432.4

SU Xia, ZHU Rui-hao, CHEN Xiao-ling, YANG Li-cong, ZHOU Hong-zhuan, XU Fu-zhou, YANG Bing. The Establishment of Gene Chip for Chicken Infectious Anemia Virus, Reticuloeotheliosis Virus and Avian Leucosis Virus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(6): 91-96. doi: 10.7668/hbnxb.2015.06.014.

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http://www.hbnxb.net/EN/Y2015/V30/I6/91

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