Characterization and Gene Mapping of Stage Thermo-sensitive Green-revertible Albino Mutant stgra254 in Rice

doi:10.7668/hbnxb.2017.03.001

Abstract

Abstract: In order to explore and identify more albino mutants for studying gene functions,a stage thermos-sensitive green-revertible albino mutant in rice,designated as stgra254,was isolated by treating the seeds of japonica variety Xiushui 09 using EMS.The phenotype of gra254 is thermos-sensitive.Under a constant temperature of 28℃,the white spots appeared on the fully expanded sixth leaves,and gradually enlarged and merged into one at maximum tillering stage.Then the leaves withered to death.The sixth leaf blades showed milder expression of the mutant phenotype under 32℃ condition than that under 28℃ condition,and gradually turned green from the third day onward.Under 24℃ condition,however,the gra254 plants produced normal green leaves.Histochemical staining analysis indicated that the albino trait of stgra254 was caused by the programmed cell death,which probably caused by oxidative burst accompanying by the accumulation of H₂O₂.Analysis by fluorescence analyzer presented that the maximal photochemical efficiency of PS Ⅱ decreased significantly.Genetic analysis indicated that the thermos-sensitive green-revertible albino trait was controlled by a single recessive nucleic gene.Based on the F₂ population derived from a cross between stgra254 and Zhenshan 97,the stgra254 was located between the SSR markers of RM17206 and RM17277 on chromosome 4 by bulked segregant analysis and linkage analysis.The genetic distance was 0.48,5.22 cM, respectively.

Key words: Rice, Green-revertible albino, Thermos-sensitive, Gene mapping

CLC Number:

ZOU Jincai, ZHANG Weilin, XIA Minghui, QIU Yangsong, WANG Changchun, YANG Ling, ZHANG Xiaoming. Characterization and Gene Mapping of Stage Thermo-sensitive Green-revertible Albino Mutant stgra254 in Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(3): 1-6. doi: 10.7668/hbnxb.2017.03.001.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2017.03.001

http://www.hbnxb.net/EN/Y2017/V32/I3/1

References

[1] Kusumi K,Sakata C,Nakamura T,et al.A plastid protein NUS1 is essential for build-up of the genetic system for early chloroplast development under cold stress conditions[J].The Plant Journal:for Cell and Molecular Biology,2011,68(6):1039-1050.
[2] 郭涛, 黄永相, 黄宣, 等.一个控制水稻叶色白化转绿及多分蘖矮秆基因hw-1(t) 的鉴定[J].作物学报,2012,38(1):23-35.
[3] Chen T,Zhang Y,Zhao L,et al.Fine mapping and candidate gene analysis of a green-revertible albino gene gra (t) in rice[J].Journal of Genetics and Genomics,2009,36(2):117-123.
[4] Yoo S C,Cho S H,Sugimoto H,et al.Rice virescent3 and stripe1 encoding the large and small subunits of ribonucleotide reductase are required for chloroplast biogenesis during early leaf development[J].Plant Physiology,2009,150(1):388-401.
[5] Qin D,Dong J,Xu F,et al.Characterization and fine mapping of a novel barley stage green-revertible albino gene (HvSGRA) by bulked segregant analysis based on SSR assay and specific length amplified fragment sequencing[J].BMC Genomics,2015,16(1):838.
[6] Qi Y, Liu X, Liang S, et al. A putative chloroplast thylakoid metalloprotease VIRESCENT3 regulates chloroplast development in Arabidopsis thaliana[J].Journal of Biological Chemistry, 2016, 291(7):3319-3332.
[7] Su N,Hu M,Wu D,et al.Disruption of a rice pentatricopeptide repeat protein causes a seedling-specific albino phenotype and its utilization to enhance seed purity in hybrid rice production[J].Plant Physiology,2012,159(1):227-238.
[8] 董华林, 费震江, 魏磊, 等.水稻苗期叶片白化转绿性状研究进展[J].湖北农业科学,2012,51(23):5241-5247.
[9] 郭涛, 黄永相, 黄宣, 等.水稻叶色白化转绿及多分蘖矮秆基因hw-1(t) 的图位克隆[J].作物学报,2012,38(8):1397-1406.
[10] Reinbothe S,Pollmann S,Springer A,et al.A role of Toc33 in the protochlorophyllide-dependent plastid import pathway of NADPH:protochlorophyllide oxidoreductase (POR) A[J].The Plant Journal:for Cell and Molecular Biology,2005,42(1):1-12.
[11] Dai X B,Cao S Q,Xu X M,et al.Study on a mutant with low content chlorophyll b in a high yielding rice and its photosynthesis properties[J].Acta Botanica Sinica,2000,42(12):1289-1294.
[12] Sugimoto H,Kusumi K,Noguchi K,et al.The rice nuclear gene, VIRESCENT 2,is essential for chloroplast development and encodes a novel type of guanylate kinase targeted to plastids and mitochondria[J].The Plant Journal:for Cell and Molecular Biology,2007,52(3):512-527.
[13] 王平荣, 王兵, 孙小秋, 等.水稻白化转绿基因gra75 的精细定位和生理特性分析[J].中国农业科学,2013,46(2):225-232.
[14] Dong H,Fei G,Wu C,et al.A rice virescent-yellow leaf mutant reveals new insights into the role and assembly of plastid caseinolytic protease in higher plants[J].Plant Physiology,2013,162(4):1867-1880.
[15] Kim H,Lee H,Oh C,et al.Postembryonic seedling lethality in the sterol-deficient Arabidopsis cyp51A2 mutant is partially mediated by the composite action of ethylene and reactive oxygen species[J].Plant Physiology,2010,152(1):192-205.
[16] Gong X,Su Q,Lin D,et al.The rice OsV4 encoding a novel pentatricopeptide repeat protein is required for chloroplast development during the early leaf stage under cold stress[J].Journal of Integrative Plant Biology,2014,56(4):400-410.
[17] Liu H, Li Q, Yang F, et al. Differential regulation of protochlorophyllide oxidoreductase abundances by VIRESCENT 5A (OsV5A) and VIRESCENT 5B (OsV5B) in rice seedlings[J]. Plant and Cell Physiology, 2016, 57(11):2392-240258.
[18] Zhang Q, Xue D, Li X, et al. Characterization and molecular mapping of a new virescent mutant in rice[J]. Journal of Genetics and Genomics, 2014, 41(6):353-356.
[19] Liu J, Wang J, Yao X, et al. Characterization and fine mapping of thermo-sensitive chlorophyll deficit mutant1 in rice (Oryza sativa L.)[J]. Breeding Science, 2015, 65(2):161-169.
[20] Deng X J, Zhang H Q, Wang Y, et al. Mapped clone and functional analysis of leaf-color gene Ygl7 in a rice hybrid (Oryza sativa L. ssp. indica)[J]. PLoS One, 2014, 9(6):e99564.

Please choose a citation manager

Content to export