Study on the Effect of Anthocyanin fRom Vaccinium uliginosum L.Resistance Avian Leukosis ViRus SubgRoup A Activity

doi:10.7668/hbnxb.2013.06.030

Abstract

Abstract: To investigate whether the anthocyanins from Vaccinium uliginosum L.( AVU) could inhibit Avian Leukosis Virus subgroup A ( ALV-A) on the DF-1 cell by in vitro studies.The anthocyanins structure of sample was i-dentified by HPLC-MS.The DF-1 cells were pretreated with the ALV-A for 1 hour and then exposed to the low dose of the extracts of the anthocyanins for an additional 24 to 96 hours.The cell viability was determined by MTT assay.The DF-1 morphological changes were observed.We found that the extracts of Vaccinium uliginosum L.contained six major anthocyanins, the one of the main component was cyaniding-3-glucoside.The 40 to 80μg/mL AVU resulted in inhibi-tion of Avian Leukosis Virus subgroup A infection and reduction of apoptosis compared with control.Moreover, we found that 60μg/mL AVU was significantly inhibited the activity of the ALV-A and increased the growth of DF-1 cells.

Key words: Vaccinium uliginosum L., Anthocyanin, DF-1 cell, ALV-A, Biological activity

CLC Number:

S853.7

ZHANG Li, LEI Yong-dong, GAI Li-li, ZHAO Xiao-yan, TONG Jun-mao, WANG Dan, MA Yue, ZHANG Chao. Study on the Effect of Anthocyanin fRom Vaccinium uliginosum L.Resistance Avian Leukosis ViRus SubgRoup A Activity[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(6): 174-180. doi: 10.7668/hbnxb.2013.06.030.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2013.06.030

http://www.hbnxb.net/EN/Y2013/V28/I6/174

References

[1] 朱文赫,徐俊杰,张巍,等.长白山笃斯越橘中花色苷不同提取工艺的研究[J].食品研究与开发,2012,33( 4): 59-63.
[2] 刘荣,赵静,王振宇,等.笃斯越桔花色苷对高脂血症大鼠血脂水平的影响[J].食品工业科技,2011,32( 5): 381-383.
[3] 柳嘉,赵竞,景浩.笃斯越桔多酚提取物成分分析及其抗氧化活性的研究展[J].食品科技,2009,34( 9): 220-224.
[4] Mazza G,Kay D,Tony C, et al.Absorption of anthocyanins from blueberies and serum antioxidant status in human subjects[J].Food Chemistry,2002,50( 26): 7731-7737.
[5] Wei G Y,Casimir C A, Joan F, et al.Effects of phenolic compounds in blueberries and muscadine grapes on HepG2 cell viability and apoptosis[J].Food Research International, 2006,39( 5): 628-638.
[6] Chaovanalikit A,Wrolstad R E.Total anthocyanins and total phenolics of fresh and processed cherries and their antioxidant properties[J].Food Chemistry and Toxicology,2004,69( 1): 67-72.
[7] Zavala G,Cheng S.Detection and characterization of avianleukosis virus in Marek's disease vaccines[J].Avian Diseases,2006,50( 2): 209-215.
[8] Zhang Q C,Zhao D M,Guo H J, et al.Isolation and Identification of a subgroup a avian leukosis vrius from imported meat-type grandparent chickens[J].Virologica Sinica,2010,25( 2): 130-136.
[9] Lupiani B,Hunt H,Silva R, et al.Identification and characterization of recombinant subgroup J avian leukosis viruses( ALV) expressing subgroup A ALV envelope[J].Virol,2000,276( 1): 37-43.
[10] Barbosa T,Zavala G,Cheng S.Molecualr characterization of three recombinant isolate of avian leukosis virus obtained from contaminated Marek's disease vaccines[J].Avian Diseases,2008,52( 2): 245-252.
[11] 刘红锦,刘小莉,周剑忠.蓝莓中花色苷提取及其抗氧化活性研究[J].江苏农业学报,2009,25( 6): 1347 －1350.
[12] 于晶,郝再彬,苍晶,等.黄酮类化合物的活性研究进展[J].东北农业大学学报, 2008,39( 12): 125 －130.
[13] 程宝鸾.动物细胞培养技术[M].广州: 中山大学出版社, 2006: 10.
[14] Chesters P M,Howes K,Petherbridge L, et al.The viral envelope is a major determinant for the induction of lymphoid and myeloid tumours by avian leukosis virus subgroups A and J, respectively[J].Gen Virol,2002,83( 10): 2553-2561.
[15] Maas R,Zoelen D V,Oei H, et al.Replacement of primary chicken embryonic fibroblasts ( CEF) by the DF-1 cell line for detection of avian leucosis viruses[J].Biologicals, 2006,34( 3): 177-181.
[16] 徐春华.MTT 法检测大豆异黄酮对癌细胞的生长抑制作用[J].中央民族大学学报: 自然科学版,2012,21( 1): 58-62.
[17] 王二雷,林松毅,刘静波,等.笃斯越桔中花青素含量分析[J].食品科学, 2007,28( 10): 460-463.
[18] 杨桂霞,范海林,郑毅男,等.笃斯越橘果实中黄酮类化合物的分离鉴定[J].吉林农业大学学报, 2005,27( 6): 643-648.
[19] 张青禅.A 亚群禽白血病病毒不同分离株的基因组和生物学特性比较[D].泰安: 山东农业大学, 2010.
[20] 陈健,孙爱东,高雪娟,等.蓝莓花青素的提取及抗氧化性的研究[J].北京林业大学学报, 2011,33( 2):126-129.
[21] Cristina A L,Barbara S H,Galli R L, et al.Anthocyanins in aged blueberry-fed rats are found centrally and may enhance memory[J].Nutr Neurosci,2005,8( 2): 111 －120.
[22] 陶欣艺,卢艳花,周文瑜,等.欧洲越桔和笃斯越桔对神经细胞氧化损伤的保护作用[J].中国临床康复,2005,33( 9): 50-53.
[23] Mahmood N,Moore P S,Tommasi ND,et al.Inhibition of HIV infection by caffeoylquinic acid derivatives[J].Antiviral Chem Chemother,1993,4( 4): 235-240.
[24] 阎祖炜,朱欣,李闻文.MTT 法、CPE 观察法用于药物细胞毒性实验的比较与分析[J].实用预防医学,2007,14( 5): 1552-1554.

Please choose a citation manager

Content to export