Cloning and Constructing of the GluB-4 Promoter from Rice

doi:10.3321/j.issn:1000-7091.2007.02.006

Abstract

Abstract: The GluB-4 promoter was amplified from Oryza sativa genome by polymerase chain reaction and cloned into pGEM-T Easy vector. Sequence analysis showed that the obtained promoter fragment 1560 nucleotides and showed homology of 99.8% with the reported GluB-4 promoter. Also, the obtained promoter fragment includes TATA-box, CAAT-box and the two cis-regulatory elements, GCN4 motif and Skn-1 motif, which are necessary to mediate endosperm expression of the GluB-4 glutelin gene. The plant expression vector pCGB4P was constructed by the GluB-4 promoter.

Key words: Oryza sativa, GluB-4 gene, Promoter, Glutelin

CLC Number:

S511.01
Q789

WANG Wei-quan, YANG Cui-ping, WANG Jing-xue, LIANG Chao, LIU Li. Cloning and Constructing of the GluB-4 Promoter from Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(2): 22-25. doi: 10.3321/j.issn:1000-7091.2007.02.006.

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