Effect of PAM on Proliferation of Lymphocytes and on Signaling Related Molecules in Peripheral Blood of Piglet

doi:10.3321/j.issn:1000-7091.2007.02.005

Abstract

Abstract: The experiment was conducted to study the effect of polysaccharide from atractylodis macrocephalae (PAM) on proliferation of lymphocytes and signaling related molecules like NO, cAMP and cGMP in peripheral blood of piglet. Effects of PAM on proliferation of peripheral blood lymphocytes of piglet were tested by MTT assay. The production of IFN-γ in supernatant were determined by ELISA; the content of cAMP and cGMP were tested by RIA. The concentration of NO was tested by deoxidization assay. The results showed that PAM insignificantly increased proliferation of lymphocytes and the contents of NO, cAMP and IFN-γ in similar dose dependent manner. In conclusion, PAM might perform its immunoregulation functions via signal transduction system in immunocytes by activating immunocytes to release NO and cAMP/cGMP and raise IFN-γ.

Key words: PAM, NO, cGA, P cGMP, IFN-γ, Immunomodulation

CLC Number:

S858.28

ZHU Nan-shan, ZHANG Bin, LI Li-li, HOU De-xing. Effect of PAM on Proliferation of Lymphocytes and on Signaling Related Molecules in Peripheral Blood of Piglet[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(2): 18-21. doi: 10.3321/j.issn:1000-7091.2007.02.005.

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References

[1] 杨翠平, 劳业兴, 吴凤薇, 等. 白术的研究进展[J]. 中药材, 2002, 25(3): 206-208.
[2] 胡庭俊, 程富胜, 陈炅然, 等. 黄芪多糖对小鼠免疫细胞信号转导相关分子的影响[J]. 畜牧兽医学报, 2005, 36(6), 616-619.
[3] 王德云, 胡元亮, 孔祥峰, 等. 中药成分对培养的鸡脾脏淋巴细胞增殖的影响[J] 畜牧兽医学报, 2005, 36(11). 1210-121.
[4] 单俊杰, 王易, 王顺春, 等. 当归多糖对小鼠脾淋巴细胞增殖及诱生IFN-γ的影响[J]. 药学学报, 2002, 37(7): 497-500.
[5] Bauer, H, Jung T, Tsikas D, et al. Nitric oxide inhibits the secretion of T helper 1-and T helper 2-associated cytokines in activated human T cells[J]. Immunology 1997, 90: 205-211.
[6] Kosonen O, Kankaanranta H, Lahde M, et al. Nitric oxidereleasing oxatriazole derivatives inhibit human lymphocyte proliferation by a cyclic GMP-independent mechanism[J]. J Pharmacol Exp Ther, 1998, 286(1): 215-220.
[7] Liew F Y. Regulation of lymphocytes function by nitric oxide[J]. Curr Opin Immunol, 1995, 7(3): 396-399.
[8] Badovinac V, Trajkovic V, Mostarica, et al. Nitric oxide promotes growth and major histocompatibility complex-unrestricted cytotoxicity of interleukin-2 activated rat lymphocytes[J]. Scand J Immunol, 2000, 52(1): 62-70.
[9] 孙大业, 郭艳林, 马力耕. 细胞信号传导[M]. 北京: 科学出版社, 1997: 52-70.
[10] 李明春, 梁东升, 许自明. 灵芝多糖对小鼠巨噬细胞cAMP含量的影响[J]. 中国中药杂志, 2000, 25(1): 41-43.
[11] 俞发, 张斌, 叶正君, 等. 螺旋藻多糖对小鼠脾细胞中环腺苷酸浓度的影响[J]. 中国医院药学杂志, 1999, 19(8): 460-462.
[12] Paludan S R. Interleukin-4 and interferon-gamma: the quinterssence of a mutual antagonistic relationship[J]. Stand J Inununol, 1998, 48: 459.
[13] 杨铁虹, 贾敏, 梅其炳. 当归多糖组分促进淋巴细胞增殖及对IL-2和IFN-γ的诱生作用[J]. 中药材, 2005. 28(5): 405-407.

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