Genetic Differences and Diversity of Mitten Crabs Eriocheir from Different Rivers based on mtDNA Analysis

doi:10.7668/hbnxb.2008.03.014

Abstract

Abstract: The genetic differences and diversity of mitten crab populations from 6 river systems in mainland China,1 population from Russia were studied based on RFLP analysis of mitochondrial cytochrome oxidase subunit I(COI)gene of 289 individual mitten crabs.The results showed that 15 composite haplotypes were detected among 11 populations after 11 restriction endonucleases(8 recognizing 4 base pairs and 3 recognizing 6 base pairs)digestion,while haplotype 1 was shared by all the samples except Eriocheir hepuensis and had high percentage(46.4%-96.7%),Eriocheir hepuensis was mainly composed of haplotype 4(91.7%)and had distinct genetic differences compared with Eriocheir sinensis from Yellow River,Haihe and Liaohe(Pnet=0.060-0.061);Genetic diversity of intrapopulation existed in Eriocheir sinensis of 6 River systems,Eriocheir hepuensis and Eriocheir japonica(π=0.000 2～0.016 7).

Key words: Mitten crab, Genetic deference, Genetic diversity, M itochondrial CO I gene, RFLP

CLC Number:

Q959.4

HU Peng-fei, LI Xiao-dong, DAI Wei, WANG Qian, WANG Xiao-mei. Genetic Differences and Diversity of Mitten Crabs Eriocheir from Different Rivers based on mtDNA Analysis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(3): 50-55. doi: 10.7668/hbnxb.2008.03.014.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2008.03.014

http://www.hbnxb.net/EN/Y2008/V23/I3/50

References

[1] 堵南山.中华绒螯蟹在欧洲[J].水产科技情报,2003,30(1):7-12.

[2] 邹曙明,李思发.中华绒螯蟹欧洲、美国的移植[J].上海水产大学学报,2002,11(4):393-396.

[3] 堵南山.中华绒螫蟹在北美洲[J].水产科技情报,2002,30(4):151-152,168.

[4] 杨培银.中华绒螫蟹种群差异研究进展[J].水产养殖,2000(5):22-23.

[5] 许加武,任明荣,李思发.长江、辽河、瓯江中华绒螯蟹种群的形态判别[J].水产学报,1997,21(3):269-274.

[6] 李晨虹,李思发.中国大陆沿海六水系绒螯蟹(中华绒螫蟹和日本绒螯蟹)群体亲缘关系:形态判别分析[J].水产学报,1999,23(4):338-342.

[7] 赵金良,李思发.中国大陆六水系绒螯蟹(中华绒螯蟹和日本绒螯蟹)群体亲缘关系:生化遗传差异分析[J].水产学报,1999,23(4):331-336.

[8] 周开亚,高志千.RAPD标记鉴别中华绒螯蟹种群的初步研究[J].应用与环境生物学报,1999,5(2):176-180.

[9] 李思发,邹曙明.中国大陆沿海六水系绒螯蟹(中华绒螯蟹和日本绒螯蟹)群体亲缘关系:RAPD指纹标记[J].水产学报,1999,23(4):325-330.

[10] 孙红英,周开亚,陆健健,等.中国大陆绒螯蟹线粒体16S rDNA序列变异与分子鉴定标记[J].自然科学进展2002,12(5):485-490.

[11] 李思发,吕国庆.长江中下游鲢鳙草青四大家鱼线粒体DNA多样性分析[J].动物学报,1998,44(1):82-93.

[12] 张四明,汪登强,邓怀,等.长江中游水系鲢和草鱼群体mtDNA遗传变异的研究[J].水生生物学报,2002,26 (2):142-147.

[13] 郑冰蓉,张亚平,昝瑞光.洱海四种鲤鱼线粒体DNA遗传相似性的初步研究[J].遗传,2001,23(6):544-546.

[14] 胡文革,王金富,盛金良,等.新疆三种雅罗鱼属鱼类mtDNA D-loop多态性及起源分化分析[J].遗传,2003,25(4):414-418.

[15] Tang B P,Zbeu K Y,Song D X,et al.Molecular systematics of the Asian mitten crabs,genus Erieeheir (Crustacea:Brachyura)[J].Molecular Phylogenetics and Evolution,2003,29(2):309-316.

[16] 张凤英,马凌波.长江、辽河、瓯江三水系中华绒螯蟹mt12SrDNA片断序列的比较[J].海洋渔业,2004,26(2):147-151.

[17] 孙红英,王光跃,张代臻,等.中华绒螯蟹与合浦绒螯蟹两地理亚种的线粒体DNA序列变异[J].动物学报,2005,51(5):862-866.

[18] Wolf C,Rentsch J,Hubner P.PCR-RFLP analysis of mito-chondrial DNA:a reliable method for species identification[J].Journal of Agricultural and Food Chemistry,1999,47 (4):1350-1355.

[19] Anirban Chakraborty,Futeshi Aranishi,Yuldo Iwatsuki.Molecular identification of hairtail species (Pisces:Trichiuri-dae)based on PCR-RFLP analysis of the mitochondriul 16S rRNA gene[J].Journal of Applied Geneties,2005,46(4):381-385.

[20] 王晓梅,宋文芹,李秀兰,等.RAPD技术检测野生鲫鱼和四个金鱼代表品种的基因组DNA多态性[J].遗传,1998,20(5):7-11.

[21] Folmer O,Black M,Heeh W,et al.DNA primers for ampli-fication of mitoebendrial cytoehrome e oxidaee sabunit I fromdiverse metazoan invertebrates[J].Molecular Marine Biology and Bioteehnolngy,1994,3:294-299.

[22] 孔晓瑜,喻子牛,刘亚军,等.中华绒螯蟹与日本绒螯蟹线粒体COI基因片段的序列比较研究[J].青岛海洋大学学报,2001,31(6):861-866.

[23] Brown W M,George M J R,Wilson A C.Rapid evolution of animal miteehondrial DNA[J].Proceedings of the National A-cademy of Sciences of the United States of America,1979,76:1967-1971.

[24] Nei M,Tajima F.DNA polymorphism detectable by restriction endonueleases[J].Genetics,1981,97 (1):145-163.

[25] Nei M,Li W H.Mathematical model for studying genetic vari-ation in terms of restriction endonucleases[J].Proceedings of the National Academy of Sciences of the United States of America,1979,76:5269-5373.

[26] Nei M,Miller J C.A simple method for estimating average number of nuclootide substitution within and between popula-tions from restriction data[J].Genetics,1990,125:873-879.

[27] 常青,周开亚.分子进化研究中系统发生树的重建[J].生物多样性,1998,6(1):55-62.

[28] Kumar S,Tamura K,Jakobsen I B,et al.MEGA2:molecular evolutionary genetics analysis software[J].Bioinformatics,2001,17(12):1244-1245.

[29] Li J,Nei M.Relative efficiencies of the maximum-parsimony and distance-matrix methods of phylogeny construction for re-striction data[J].Molecular Biology and Evolution,1991,8 (3):356-365.

[30] 杜宗汉.浅议不同水系河蟹养殖效果和鉴别方法[J].水产养殖,1996(1):26-27.

[31] 王丹,于伟君.辽河长江两水系中华绒螯蟹脂酶和乳酸脱氢酶的同工酶比较研究[J].辽宁大学学报:自然科学版,1995,2(4):79-81.

[32] 张洁,陈立侨,周忠良,等.中华绒螯蟹同工酶发育遗传学研究Ⅰ,长江中华绒螯蟹不同组织同工酶的特异性分析[J].华东师范大学学报,1996(动物学专集):32-42.

[33] 邱涛,陆仁后,项超美,等.RAPD方法对中华绒螯蟹长江、辽河、瓯江三群体的遗传多样性分析[J].淡水渔业,1997,27(5):2-6.

[34] 高志千,周开亚.中华绒螯蟹遗传变异的RAPD分析[J].生物多样性,1998,6(3):186-190.

[35] Hedgecock D,Traeey M L,Nelson,K.The Biology of Crus-tacea[M]// Abele L G.Genetic.New York:Academic Presa,1982:283-403.

[36] Li G,Shen Q,Xu Z X.Morphometrie and biochemical genetic variation of the mitten crab,Eriocheir,in southern China[J].Aqnaeulture,1993,111:103-115.

[37] Lu R H,Qiu T,Xiang C M,et al.RAID and AFLP tech-niques for the analysis of genetic relationships in two genera of Decapoda[J].Crnstaceana,2000,73:1027-1036.

[38] 谢浩,陆仁后,项超美,等.利用RAPD技术对三种绒螯蟹亲缘关系的研究[J].水生生物学报,1999,23(2):120-126.

[39] 张列士,姜治忠,李军.日本绒螯蟹与不同水系中华绒螯蟹的形态比较[J].上海水产大学学报,2002,11 (2):110-113.

[40] Nelson K,Hedgeeock D.Enzyme polymorphism and adaptive strategy in the Decapod Crnstaea[J].American Naturalist,1980,116(2):238-279.

[41] 王晓梅,邢克智,戴伟,等.不同水系中华绒螯蟹线粒体COI基因片段的RFLP分析[J].华北农学报,2007,22(1):78-82.

Please choose a citation manager

Content to export