Molecular Cloning and Sequence Analysis of Ovine Gastorkine-1

doi:10.7668/hbnxb.2008.01.009

Abstract

Abstract: In order to clone and analysis the ovine Gastorkine -1, the known sequence of bovine Gastorkine -1(NM001001148)was selected to search the GenBank ovine EST database, from which two ESTs(EE780384, EE77958)were obtained. Based on the sequence spliceosome, a pair of primers in 5 c -UTR and 3c -UTR was designed and used toamplify the total RNA extracted from mucosal tissue of ovine abomasum by RT -PCR,then the PCR products were clonedand sequenced.The results showed that ovine Gastorkine -1 sequence shared 8210%, 4818%, 8514% and 9619% sim- ilarity with the corresonding genes of human,mouse, pig and cattle, respectively.The signal peptide of predicted amino acidsequence contained 1- 20 aa and the structural domain BRICHOS had 54- 150 aa, presenting the same structural featureto those of human,mouse, pig and cattle.

Key words: Sheep, Gastorkine -1, Molecular cloning, Sequence analysis

CLC Number:

S811.3
Q789

QIAO Xin-an, YANG Guo-yu, WANG Yan-ling, WANG Yue-ying, HAN Li-qiang, ZHU Yan-cai, DU Jun-xia, FAN Pei. Molecular Cloning and Sequence Analysis of Ovine Gastorkine-1[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(1): 38-40. doi: 10.7668/hbnxb.2008.01.009.

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