Influence of Lipoic Acid on Transformation of Cucumber Cotyledon in vitro by Agrobacterium tumefaciens

doi:10.3969/j.issn.1000-7091.2012.z1.010

Abstract

Abstract: It's difficult to increase the transformation efficiency in cucumber． The effect of LA and AS duringtransformation of cucumber cotyledon node was investigated． The plant regenerated efficiency of cucumber was significantly increased from 28． 3 % to 86． 7% ， the transformation efficiency was 7． 5% analyzed by Southern-blotwhen 100 mg /L LA and 50 mg /L AS added in culture media and Agrobacterium liquid． Including lipoic acid in culture media during Agrobacterium transformation processes of cucumber can improve the transformation method． LAapplication in transformation has enabled the resolution of problems in cucumber: difficulty in regenerating transformed tissues and shoot escapes that limit the number of transgenic plants．

Key words: Lipoic acid, Agrobacterium tumefaciens, Transformation, Cucumber, Cotyledon node．

CLC Number:

S642.2

WANG Ye, GU Xing-fang, ZHANG Sheng-ping, MIAO Han. Influence of Lipoic Acid on Transformation of Cucumber Cotyledon in vitro by Agrobacterium tumefaciens[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.3969/j.issn.1000-7091.2012.z1.010.

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