Semi-quantitative RT-PCR Method of Detection of Heat Stress on Fish Liver Content of HSP70 mRNA

doi:10.7668/hbnxb.2010.S1.023

Abstract

Abstract: This research investigated the variation of HSP70 mRNA content in the liver of Carassius auratus at different time lapses after heat stress processing by using semiquantitative RT-PCR. Two primers were designed for this study according to the sequences of Carassius auratus's heat shock protein70(HSP70) and beta-actin mRNA published by GenBank. The results show that the HSP70 expression in liver tissue is time-dependent by showing an increase-to-decline trend. The expression starts to increase 1 hour after inducing and reaches a maximum 4 h later.Subsequently,it begins to decline continuously and eventually returns to its initial value until 48 h later.

Key words: Carassius auratus, Semiquantitative RT-PCR, Heat shock, Heat shock protein 70

CLC Number:

S917

SU Ling, LI Shao-wu, WANG Di, LIU Hong-bai, LU Tong-yan, YIN Jia-sheng. Semi-quantitative RT-PCR Method of Detection of Heat Stress on Fish Liver Content of HSP70 mRNA[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(S1): 100-104. doi: 10.7668/hbnxb.2010.S1.023.

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