The Effects of Thyroxine and Glucagon on Heat Shock Proteins Expression of Bovine Mammary Epithelial Cells under Heat Circumstance in vitro

doi:10.7668/hbnxb.2010.06.033

Abstract

Abstract: The experiment was conducted to study the effects of thyroxine(T4)or glucagon on heat shock proteins( HSPs)expression of bovine mammary epithelial cells cultured under heat circumstance in vitro.Bovine mamma. ry epithelial cells were cultured with different concentration of T4 or glucagon, which were exposed at 42%as heat treatment model for 12 h.Then mRNA transcript and protein expression were detected by RT—qPCR and ELISA.The resuhs showed as follows：Every experimental concentration of T4 and glucagon can significantly decrease the mRNA transcript level of HSP27, HSP70, HSP90, HSF·1(P<0.05), which ceils cultured at 42。C；The protein expression can also decreased extremely markedly due to cells cultured with different concentration of glucagon at 42%；at the same time, every experimental concentration of T4 can significantly decrease the protein expression of HSP27, HSP70 at 420C(P<0.01), but only 0.01, 0.1, 2 p。mol/L of T4 had the significantly effects on HSP90(P<0.01).In conclusion, T4 and glucagons can decrease HSPs mRNA transcript and protein expression level of cells cultured under heat circumstance.

Key words: Thyroxine(y4), Glucagon, Bovine mammary gland epithelial cells, Heat shock proteins(HSPs)

CLC Number:

S823

CUI Rui-lian, WANG Jia-qi, BU Deng-pan, WEI Hong-yang, HU Han, ZHOU Ling-yun. The Effects of Thyroxine and Glucagon on Heat Shock Proteins Expression of Bovine Mammary Epithelial Cells under Heat Circumstance in vitro[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(6): 173-177. doi: 10.7668/hbnxb.2010.06.033.

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