Ca2+CaM Pathway of Heat Shock Signal Transduction

doi:10.3321/j.issn:1000-7091.2003.z1.007

Abstract

Abstract: Laboratory studies by the author show ed the involvement of calcium and calciumactivatedcalmodulin.(Ca²⁺-CaM) in heat shock.HS) signal transduction..sing Fluo3/ AM and Laser Scanning Confocal Microscopy,it was found that the increase of int racellular free calcium ion concent ration started within.minafter.37 HS in.heat.The levels of CaM mRNA and protein increased during HS at 37 in the presenceof Ca²⁺.The expression of hsp26 and hsp70 genes,synthesis of heat shock proteins were upregulated by addition of CaCl2,and dow nregulated by the Ca²⁺ chelator EGTA,the Ca²⁺ channel blockers LaCl3 and verapamil,or the CaM antagonists W7,CPZ and TFP. The acquired thermotolerance developed by HS at 37 was eliminated by treatment with EGTA,verapamil,CPZ and TFP. The temporal ex pression of the CaM1-2gene,and the hsp26 and hsp70 genes,demonst rated that CaM is located upstream in HS signal transduction,and Ca²⁺ is the key factor turning on HS signal transduction pathw ay. The result sobtained by gel mobility shift assaydemonst rated that Ca²⁺ CaM is involved in HS signal transduction by regulat ing DNAbinding activity of HStranscription factor. Based on above findings,.new pathw ay,Ca²⁺ CaM pathw ay of HS signal transduction,was proposed.

Key words: Calcium ion, Calmodulin, Heat shock proteins, Heat shock t ranscript ion factor, Signal transduction

CLC Number:

S51.01??

ZHOU Ren-gang. Ca²⁺CaM Pathway of Heat Shock Signal Transduction[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.3321/j.issn:1000-7091.2003.z1.007.

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