Cloning and Expression Analysis of TaCML79 in Wheat

doi:10.7668/hbnxb.2016.06.001

Abstract

Abstract: In order to study the function and molecular mechanism of wheat calmodulin-like protein gene(CML) in plant stress response,one CML gene TaCML79 was cloned in wheat based on electron cloning and RT-PCR.Sequence analysis showed that the open reading frame (ORF) of TaCML79 was 588 bp,encoding 195 amino acids of protein containing two typical and one incomplete EF-hand domains,with predicted molecular weight of 20.45 kDa and isoelectric point of 4.6.Multiple sequence alignment and phylogenetic tree analysis showed that TaCML79 was closely related to the ObCML35 and OsCML10,with 84.6% and 86.8% identity respectively.The expression of TaCML79 was induced or inhibited by heat shock,NaCl,osmotic and cold stress in root and shoot tissue,suggesting that TaCML79 might be closely related to plant resistance to abiotic stress.

Key words: Triticum aestivum, Calmodulin-like proteins, EF-hand domain, Gene cloning, Gene expression

CLC Number:

Q78
S512.1

ZHOU Shuo, LIU Yongwei, DONG Fushuang, YANG Fan, ZHAO He, CHAI Jianfang, LÜ Mengyu, SUN Guozhong, WANG Haibo. Cloning and Expression Analysis of TaCML79 in Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(6): 1-6. doi: 10.7668/hbnxb.2016.06.001.

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