Mongolian Almond Ca2+ Binding Protein Encoding Gene Systemic Identification and Expression Analysis

doi:10.7668/hbnxb.2017.02.013

Abstract

Abstract: Mongolian Almond (Prunus mongolica Maxim)belongs to Rosaceae,under national protection of second class,primarily distributes at the Northwestern Dessert Region of China and is tolerant to drought and poor soil.Calcium ion is the most important signaling molecule for plant sensing and resistance to environment stresses.In this study,based on transcriptome analysis of the leaf,we identified sixteen strings of full length transcripts,including four Ca²⁺ ATPase (ECA/ACA),three novel Ca²⁺ channel protein with several transmembrane domains (ERD),five Ca²⁺/H⁺ antiporter (CAX),two Ca²⁺ dependent protein kinase (CPK)and two calmodulins (CAM).Their predicated structures are similar to those homologies in Arabidopsis.Four single genes from the ERD,CAX,CPK and CAM categories,respectively,had obvious expression in the leaves and roots,among which the ERD,CPK and CAM genes expression had been regulated by drought and salt at different extent.It indicated that the genes might modulate the plant's environmental stress-tolerant ability.

Key words: Mongolian almond, Transcriptome, Ca²⁺ binding protein, Calmodulin

CLC Number:

S662.9

LIU Yang, TIE Ying, YANG Jia, QI Zhi, MAO Huiping. Mongolian Almond Ca²⁺ Binding Protein Encoding Gene Systemic Identification and Expression Analysis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(2): 81-86. doi: 10.7668/hbnxb.2017.02.013.

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References

[1] Kudla J,Batistic O,Hashimoto K.Calcium signals:the Lead currency of plant information processing[J].The Plant Cell,2010,22(3):541-563.
[2] 高天,铁英,王妍,等.拟南芥低钙诱导cDNA文库的构建[J].北方农业学报,2016,44(5):8-14.
[3] Reddy A S,Ali G S,Celesnik H,et al.Coping with stresses:roles of Calcium-and Calcium/Calmodulin-regulated gene expression[J].Plant Cell,2011,23(6):2010-2032.
[4] 张胜博,刘景辉,李立军,等.燕麦AsNAC1耐盐基因的克隆及初步验证[J].北方农业学报,2016,44(5):1-7.
[5] Robertson D.Modulating plant calcium for better nutrition and stress tolerance[J].ISRN Botany,2013,2013(5):1-22.
[6] 呼斯乐,白晨,张惠忠,等.转基因作物检测技术研究进展[J].内蒙古农业科技,2014(5):98-101.
[7] Ma T,Wang J,Zhou G,et al.Genomic insights into salt adaptation in a desert poplar[J].Nat Commun,2013,4(7):2797.
[8] Pang T,Ye C Y,Xia X,et al.De novo sequencing and transcriptome analysis of the desert shrub,Ammopiptanthus mongolicus,during cold acclimation using Illumina/Solexa[J].BMC Genomics,2013,14(1):488.
[9] Bhardwaj J,Chauhan R,Swarnkar M K,et al.Comprehensive transcriptomic study on horse gram(Macrotyloma uniflorum):De novo assembly,functional characterization and comparative analysis in relation to drought stress[J].BMC Genomics,2013,14(1):647.
[10] Ma X,Wang P,Zhou S,et al.De novo transcriptome sequencing and comprehensive analysis of the drought-responsive genes in the desert plant Cynanchum komarovii[J].BMC Genomics,2015,16(1):753.
[11] Li H E,Yao W J,Fu Y R,et al.De Novo assembly and discovery of genes that are involved in drought tolerance in tibetan sophora moorcroftiana[J].PLoS One,2015,10(1):e111054.
[12] Zhang L,Hu X,Miao X,et al.Genome-Scale transcriptome analysis of the Desert Shrub Artemisia sphaerocephala[J].PLoS One,2016,11(4):e0154300.
[13] 黄小鹏,斯琴巴特尔,吴榕.阿拉善荒漠区蒙古扁桃的种群龄级结构与空间分布格局[J].内蒙古林业科技,2014,40(1):7-11.
[14] 马松梅,聂迎彬,段霞.等蒙古扁桃植物的潜在地理分布及居群保护优先性[J].生态学报,2015,35(9):2960-2966.
[15] 李刚,王科.东大山林区蒙古扁桃的调查与保护对策研究[J].甘肃科技,2015,31(7):138-140.
[16] 杨跃文,季蒙,刘新前,等.内蒙古贺兰山自然保护区不同生境下蒙古扁桃种群结构与空间分布[J].山地学报,2015,33(1):42-47.
[17] 张杰,王佳,李浩宇,等.濒危植物蒙古扁桃不同地理种群遗传多样性的ISSR分析[J].生态学报,2012,32(14):4443-4452.
[18] 杜巧珍,红雨.包贺喜图.珍稀濒危植物蒙古扁桃研究进展[J].内蒙古师范大学学报:自然科学汉文版,2010,39(3):308-312.
[19] 梅曙光,张国强,朱玉安,等.蒙古扁桃育苗技术研究[J].宁夏农林科技,2011,52(9):29-30.
[20] 哈伟贵.蒙古扁桃在海原南华山的人工繁殖育苗及栽培技术探讨[J].现代园艺,2014(20):24-24.
[21] 耿军,朱立新,贾克功.蒙古扁桃单芽茎段培养体系的建立[J].西北农业学报,2007,16(5):218-221.
[22] 吴建华,王立英,李健.蒙古扁桃的组织培养与快速繁殖技术研究[J].安徽农业科学,2010,38(4):1733-1734.
[23] 王进,张勇,赵刚,等.蒙古扁桃种子萌发和幼苗生长对渗透胁迫的响应[J].中国园艺文摘,2015,32(8):236-236.
[24] 金丽萍,崔世茂,杜金伟,等.干旱胁迫对不同生态条件下蒙古扁桃叶片PAL和C_4H活性的影响[J].华北农学报,2009,24(5):118-122.
[25] 王琚钢,高晓敏,白淑兰,等.丛枝菌根对蒙古扁桃抗旱性影响研究[J].干旱区资源与环境,2014,28(12):138-142.
[26] Wang J G,Zheng R,Bai S L,et al.Mongolian almond(Prunus mongolica maxim):the Morpho-physiological,biochemical and transcriptomic response to drought stress[J].PLoS One,2015,10(4):e0124442.

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