Abstract: Using homology cloning method,the full-length cDNA of galactinol synthase gene VvGolS2, VvGolS3 and VvGolS4 were cloned from Vitis vinifera cultivar Zuoyouhong tissue culture seedling.The analysis of VvGolS2, VvGolS3 and VvGolS4 sequencing indicated that three genes were 954,978 and 1 011 bp ranging in size,coded as 317,325,336 amino acids respectively with molecular weight were 36.65,36.97,38.12 kDa and the isoelectric point were 5.12,5.33,5.16 respectively.Three VvGolSs all possessed a conserved hydrophobic APXAA pentapeptide domain in the C-terminal region.Real-time PCR analysis showed that three VvGolSs were expressed different in all tested tissues,with the highest expression of VvGolS2 and VvGolS4 in leaves,and VvGolS3 was highly expressed in flowers and tendrils. Using tissue culture seedling,the relative expression levels of VvGolS2, VvGolS3 and VvGolS4 after different stresses or different stress singling factors treatment were checked.The VvGolS2 and VvGolS4 were highly induced by salt stress,while the VvGolS3 was only induced by low temperature.Moreover, VvGolS2 was significantly induced by stress-related signal molecules,such as abscisic acid (ABA),ethylene (ACC),and hydrogen peroxide (H₂O₂).The VvGolS4 was induced by ACC,H₂O₂ and H₂S,and the VvGolS3 was induced by ABA and ACC.Together,these results suggested that these three VvGolSs were involved in abiotic stresses resistance in grape with different functions.

Key words: Vitis vinifera, Galactinol synthase gene, Gene clone, Expression analysis

CLC Number: 

• Q78