Clone and Expression Analysis of CkLEA4-1 from Caragana korshinskii Kom.

doi:10.7668/hbnxb.2017.02.015

Abstract

Abstract: In order to research the biological function of the LEA protein,a gene encoding LEA protein from the suppression subtractive hybridization library of Caragana korshinskii Kom.was screened and cloned by using RT-PCR method.The open reading frame (ORF)of the gene length was 1 119 bp,encoded a protein of 373 amino acids,named CkLEA4-1.Based on sequence alignment and phylogenetic analysis,it was inferred that CkLEA4-1 belonged to group 5 late embryogenesis abundant protein (LEA).The expression of CkLEA4-1 under high salt and drought conditions were detected by Real-time quantitative PCR,the results showed that CkLEA4-1 could be induced at different levels,which suggested that the gene might be involved in stress response of Caragana korshinskii Kom..The over-expression vector pCanG-CkLEA4-1 of CkLEA4-1 was constructed successfully,and the transgenic homozygous lines were obtained,which provided materials for the further study of CkLEA4-1 gene function in Caragana korshinskii Kom.

Key words: Caragana korshinskii Kom., LEA, Gene cloning, Expression analysis

CLC Number:

S793.3

HUA Lubin, YU Xiumin, YANG Qi, WANG Ruigang, BAO Lide. Clone and Expression Analysis of CkLEA4-1 from Caragana korshinskii Kom.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(2): 96-103. doi: 10.7668/hbnxb.2017.02.015.

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