Effects of Recombination HSP70 on Immune Function of Tilapia Peripheral Blood Lymphocyte

doi:10.7668/hbnxb.2011.S2.045

Abstract

Abstract: In this report,we use the recombinant tilapia Hsp70(tHsp70)from Pichia yeast to prepared non-covalent HSP70-antigen complexes with thallus and extracellular bacteria products(ECP)of Streptococcus iniae,and investigated their effects on cellular immune response of tilapia by detection of Proliferation and related immune gene expression of peripheral blood lymphocyte in vitro.Our results demonstrated that tHsp70 could form complexes with thallus and ECP of tilaplia in vitro.In addition,tHsp70(10 μg/mL)and all tHsp70(10 μg/mL)-thallus complexes enhanced the proliferation of tilapia peripheral blood lymphocytes(P0.01).Similarly,thallus alone also enhanced the proliferation of tilapia peripheral blood lymphocytes(P0.05).By contrast,ECP alone exhibited some inhibitory effect on the proliferation of tilapia peripheral blood lymphocytes(P=0.056).However,the complex of tHsp70 at 10,but not at 100 and 1 μg/mL,with ECP reduced the inhibitory effect of ECP on the proliferation of tilapia peripheral blood lymphocytes.Moreover,tHsp70(10 μg/mL)alone or tHsp70(10 μg/mL)-antigen complexes could significantly influence the expression of immune related genes(PGK,MMP9,ICER,MHCⅡ)in tilapia peripheral blood lymphocyte compare to antigen alone and medium control groups.The study indicated that tHsp70 have the immunoadjuvant and the immunopotentiator function,and provided experimental data for development the tHsp70 based vaccine against tilapia streptococcal diseases.

Key words: Heat shock protein 70, Lymphocyte, Immune function, Streptococcus iniae, Vaccine

CLC Number:

S965.125

CHEN Ming, WANG Qiu-hua, WANG Rui, GAN Xi, LI Li-ping, LEI Ai-ying, LIANG Wan-wen, HUANG Wei-yi. Effects of Recombination HSP70 on Immune Function of Tilapia Peripheral Blood Lymphocyte[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(S2): 213-218. doi: 10.7668/hbnxb.2011.S2.045.

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