Abstract: Fruit of apple (Malus domestica Borkh．cv．Gala) was used to analyses cell wall metabolism and the?related enzymes gene expression and the regulation by 1-MCP，ethephon and cold storage． The results indicated that?fruit firmness had significantly correlated with cell wall components，which was positive to WSP and negative to CSP?and hemi-cellulose，but little relationship with ISP during fruit softening in Gala apple． Both 1-MCP treatment and?cold storage significantly inhibited the increase in WSP content，prevented CSP and hemi-cellulose from degradation． Among the cell wall enzymes，β-Gal activity was the highest and rapidly increased，and its gene expression also?sharply enhanced during fruit softening． Although the increase rate in α-L-Af was no more than β-Gal，both two enzymes had a similar trend in activity and gene expression and strongly inhibited by 1-MCP treatment and cold storage． However，PG and PME activity and gene expression could not be completely inhibited by 1-MCP treatment and?cold storage though they were showed an increase trend． The cell wall enzyme activity had significant negative correlation with fruit softening in Gala apple，and their correlation was dramatically affected by 1-MCP and low temperature． Otherwise，the promotion by ethephon treatment was no more than the inhibition by 1-MCP treatment and cold?storage in cell wall enzymes activity and gene expression in Gala apple fruit．

Key words: Malus×domestica Borkh． cv． Gala, Cell wall metabolism, Enzymes activity, Gene expression, Fruit softening

CLC Number: 

• S661.01

• Q78