Correlations Between SNP of PRL/PRLR Genes and Production Cashmere Traits on Inner Mongolion White Cashmere Goat

doi:10.3969/j.issn.1000-7091.2012.02.012

Abstract

Abstract: Ardrs white cashmere goats were used as experimental sample，and partial sequence of PRL and?PRLR genes were used as candidate gene to research SNP by PCR-SSCP and DNA sequencing technologies. Results?were analyzed by least square mean (LSM) between production traits of different genotype. Relationship of SNP of?PRL and PRLR genes and production cashmere traits were studied to find molecular marker of MAS. There were SSCP polymorphism at P1 of PRL and P3 of PRLR，and existed in C-A mutation at X76049:g. 576 bp of Exon 5 of?PRL gene，which resulted in CCC-ACC of the 176thcodon from Pro into Thr. The results showed that there was the?most significant correlation with cashmere thickness at polymorphism site(P ＜0. 01)，and cashmere thickness of CA?genotype increased by 0. 674 cm compared with that of CC;there was T-C mutation at HQ266606:g Exon 10 of?PRLR gene，which lead to CTT-CCT of the 359thcodon from Lue into Pro，of which polymorphism was significant?difference (P ＜0. 05) to cashmere fineness，and cashmere fineness of AA genotype decrease by 0. 38 μm compared?with that of BB. P1-CA was the favorable marker genotype for cashmere thickness trait，while P3-AA was the marker?genotype of cashmere fineness trait，which acted as molecular marker of MAS for production cashmere traits.?

Key words: Cashmere goat, PRL/PRLR gene, SNP, Production cashmere traits

CLC Number:

LIU Bin, LI Yu-rong, MA Yue-jun, ZHAO Cun-fa, WEI Guang-rong, LI Jin-quan. Correlations Between SNP of PRL/PRLR Genes and Production Cashmere Traits on Inner Mongolion White Cashmere Goat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(2): 61-66. doi: 10.3969/j.issn.1000-7091.2012.02.012.

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