ACTA AGRICULTURAE BOREALI-SINICA ›› 2018, Vol. 33 ›› Issue (5): 23-28. doi: 10.7668/hbnxb.2018.05.004

Special Issue: Animal husbandry Biotechnology

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Assembly and Analysis on Mitochondrial Genome Sequence in Inner Mongolia Cashmere Goat(Alashan Type)

SU Rui1,4, YANG Zhong2, LI Chun3, LI Xiaokai1, MA Yuhao1, LI Jinquan1,4   

  1. 1. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
    2. Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture, Hohhot 010018, China;
    3. Hohhot Center for Control and Prevention, Hohhot 010070, China;
    4. College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao 028000, China
  • Received:2018-04-15 Published:2018-10-28

Abstract: In order to study the Inner Mongolia Cashmere goat mitochondrial genome sequence composition and the phylogenetic relationship of different varieties of goats,ensure the origin,evolution,differentiation of different groups and the formation mechanism of specific genetic characteristics of cashmere goats,we chose Inner Mongolia Cashmere goat (Alashan type) as object and sequenced the mitochondrial genome. The results showed that totally 2 551 175 100 bp base was got and Q20 was 95.40%. The construction quality of the library was good,the accuracy of the sequencing results was high,and the complete closed loop mitochondrial genome of 16 642 bp was obtained. The content of GC was 39.17% and K-mer was 53 bp. The mitochondrial genome sequence information of other varieties was downloaded from NCBI to carry out phylogenetic analysis. The results showed that the distance between 3 groups of Inner Mongolia Cashmere goats was close. The relationship between Inner Mongolia Cashmere goat (Alashan type) and Inner Mongolia Cashmere goat (Erlangshan type) was the closest,and the next one was Inner Mongolia Cashmere goat (Aerbasi type). Inner Mongolia Cashmere goat and Liaoning Cashmere goat could be divided into two groups. The outer range of San Clemente was the farthest.

Key words: Cashmere goat, Mitochondrional, Genome

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Cite this article

SU Rui, YANG Zhong, LI Chun, LI Xiaokai, MA Yuhao, LI Jinquan. Assembly and Analysis on Mitochondrial Genome Sequence in Inner Mongolia Cashmere Goat(Alashan Type)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(5): 23-28. doi: 10.7668/hbnxb.2018.05.004.

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