Abstract: In order to compare and analyze the relationship between OsRSR1 expression,sequence and functional variation and trait genetic variation. Indica rice varieties with high amylose content(AC),glutinous rice varieties with very low AC,Japonica parents and hybrid progenies with similar genetic background and significantly different amylose content were used in this study. Cloning,expression assay and bioinformatics analysis of transcription factor gene OsRSR1 were carried out. The results showed that OsRSR1 expression level was high at the initial and late stage of grain filling,but low in the middle stage,like a V-shape. OsRSR1 expression level was high in varieties with low AC,vice versa in varieties with high AC. It indicated the negative relationship between OsRSR1 expression and grain AC,and transgressive variation in hybrid progenies. DNA sequence,transcript and protein sequence of OsRSR1 were not identical. DNA base changes were detected in exons and introns of sexual hybrid progenies,and single base substitution(SBS)occurred in transcript to produce code variation,this was an important approach to genetic variation and gene polymorphism. OsRSR1 is composed of eight exons and seven introns. The changes of SBS and amino acid did not alter OsRSR1 gene structure and relative motif as well as function,which implied AP2 domains was highly conserved. The results would be helpful for further study on the molecular regulation of gene transcriptional level and genetic variation mechanism.

Key words: Rice, Transgressive variant, OsRSR1 gene sequence, Expression characteristics, Genetic variation

CLC Number: 

• Q78

• S511.03