Abstract: Temperature is very important for growth and development of rice and yield production,and it is necessary to further define the molecular mechanism of rice response to low temperature provides a theoretical basis for crop breeding resistant to cold stress. In this study,japonica rice variety Zhonghua-11 was used as a test material under the condition of low temperature treatment at 17℃,a new generation of high-throughput sequencing methods-transcriptome sequencing (RNA-Seq) analysis. Through the transcriptome analysis,the clean high-throughput reads were 46 348 074 and 52 483 052,respectively,and 2 044 differentially expressed genes were identified and screened,of which 1 252 genes were up-regulated and 792 genes were down-regulated. GO annotation analysis showed that all the differential genes were divided into three major categories:biological processes,cellular components and molecular functions,each accounting for 63.29%,8.81%,and 27.90%. We used the KEGG database to analyze the number and location of differentially expressed genes in low-temperature-regulated two-pathway photosynthetic pathway and phenylalanine metabolic pathway,and predicted the function of novel genes and interaction proteins related to photosynthesis and phenylalanine metabolism. The results showed that the low temperature stress on rice plays an important role in biological processes,especially on photosynthesis,secondary metabolism biosynthesis,and phenylalanine metabolism. Also,differentially expressed WRKY transcription factors will provide direction and basis for further study of cold tolerance mechanism.

Key words: Rice, Seedling stage, Low-temperature, Transcriptome

CLC Number: 

• Q78

• S511.03