Abstract: Sugar beet resistant and susceptible materials were used to compare the yield and quality in the field. Three different methods of protein extraction were compared,which included TCA/acetone precipitation method,soluble protein extraction method and phenol extraction method. After the treatment of viral stress by water culture,the different protein spots were analyzed by Image Master 2D Platinum Trial 5.0 software. The results showed that TCA/acetone precipitation method had better extraction effect. The comparison of field yield and quality showed that the growth of resistant materials were better than that of susceptible materials at seedling stage and late growth stage,and the yield and sugar content after harvest were higher than those of susceptible materials,thus the resistant materials performed well. The protein was analyzed by Image Master 2D Platinum Trial 5.0 software after two-dimensional electrophoresis,and 78 protein spots with obvious difference were deducted. A total of 34 active protein IDs were identified. Compared by NCBI and The Beta vulgaris Resource database Blast,the main functions of proteins expressed by disease 1 and disease 2 were photosynthesis,glycometabolism,respiratory antioxidation,signal transduction,lipid metabolism and some others. While disease 5 and disease 6 mainly expressed photosynthesis protein and respiratory antioxidant protein which were the proteins expressed by self stress reaction.

Key words: Sugar beet, Rhizomania, Two-dimensional electrophoresis, Mass spectrometry, Differential protein

CLC Number: 

• Q78

• S566.03