Abstract: Based on the results of previous study on columnar apple using RNA-seq technology，two full-length?cDNA sequences of homologous gene of MADS-box from columnar apple were cloned by RT-PCR，which named as?MdSOCla and MdSOClb． The MdSOCla contains an 708 bp open reading flame (ORF) corresponding to a deduced?protein of 235 amino acids，while the estimated molecular weight and isoelectric point (pI) of the putative protein?were 26． 95 kDa and 9． 13． The MdSOClb with an open reading flame (ORF) of 717 bp，encoding 238 amino acids?with a predicted molecular mass of 27． 49 kDa and a pI of 9． 61． The identity of nucleotide between MdSOCla and?MdSOClb was 83． 49%，and it was 73． 72% was deduced amino acid sequence． The results indicated that MdSOCla?and MdSOClb had 1 to 75 amino acids highly conserved MADS-box domain，105 to 170 for the K-box domain． The?phylogenetic tree of SOC1 showed that MdSOCla and MdSOClb were closest with apple SOC1，followed by orange?SOC1，and the evolutionary relationships of homologous genes in Arabidopsis SOC1 was farthest． Apple，orange and?sinningia were clustered together，while soybean，cowpea，grape，Arabidopsis thaliana，strawberries and magnolia were?clustered to another group． Predicted protein secondary structure showed that MdSOCla protein had the structure with?8 α-helix，5 β-sheet region，13 β-turn;and MdSOClb protein had 11 α-helix，7 β-sheet region and11 β-turn． Those results speculated that MdSOCla and MdSOClb played a different role in regulation in flower development．

Key words: Columnar apple, MADS-box, SOCl gene, Molecular cloning

CLC Number: 

• S661.1