Genome-Wide Association Study of Salt Tolerance Traits in Sunflower at Seedling Stage

doi:10.7668/hbnxb.20196164

Abstract

Abstract:

Sunflower is an important oil crop with strong salt tolerance in China.However,the production of sunflower is severely restricted by the intensification of salinization in main producing areas.To understand the salt tolerance mechanism of salt tolerance-related traits at the seedling stage of sunflower,and to identify SNP loci and candidate genes significantly associated with salt tolerance at the seedling stage of sunflower,124 sunflower germplasm accessions were used as materials.Under the stress of 250 mmol/L NaCl,genome-wide association analysis and mining of salt-tolerance loci/genes were performed based on 5 traits including plant height,leaf area,aboveground fresh weight,underground fresh weight,and SPAD value.The results showed that after 14 days of salt stress,the five trait indexes of relative plant height,relative leaf area,relative fresh weight of the above-ground part,relative fresh weight of the underground part and relative SPAD value at seedling stage had significant variations and normal distributions,and the most obvious influences were the relative fresh weight of the underground part and relative leaf area (with a coefficient of variation of over 46.57%).Eighteen significantly-associated SNP loci were obtained by genome-wide association analysis,among which Chr35448-18789497 was detected in several salt-tolerant traits;gene searches were conducted at 80 kb each in the upstream and downstream of associated loci,45 related candidate genes were screened out.Through scanning and gene annotation analysis of the associated locus intervals,four candidate genes that might be related to the salt tolerance trait of sunflower seedlings were discovered.A genome-wide association analysis was conducted using the phenotypes of salt tolerance identification at the seedling stage and genomic SNP data for sunflower germplasm accessions.SNP loci significantly associated with salt tolerance at the seedling stage were detected,and candidate genes related to salt tolerance at the seedling stage of sunflower were selected,which laid the foundation for the subsequent verification of salt-tolerant genes.

Key words: Sunflower, Germplasm resource, Salt tolerance at seedling stage, Genome-wide association study(GWAS), Gene

CLC Number:

WU Jiajun, LI Yuxiao, ZHANG Xinrong, ZHU Zirong, TAN Meilian, WANG Lei, WANG Ling, WANG Wei, ZHANG Yuxue. Genome-Wide Association Study of Salt Tolerance Traits in Sunflower at Seedling Stage[J]. Acta Agriculturae Boreali-Sinica, 2026, 41(1): 71-78. doi: 10.7668/hbnxb.20196164.

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References 34

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观测指标性状 Traits of observed index	最小值/% Min	最大值/% Max	平均值/% Mean	标准差 s	变异系数/% CV
相对株高 Relative plant height	9.40	86.44	45.37	0.15	32.63
相对SPAD值 Relative SPAD value	12.14	195.61	111.08	0.42	37.68
相对地上部分鲜质量 Relative above-ground fresh weight	8.85	64.85	40.69	0.13	31.12
相对地下部分鲜质量 Relative under-ground fresh weight	4.01	60.25	26.71	0.14	50.93
相对叶面积 Relative leaf area	1.96	54.49	25.66	0.12	46.57

观测指标性状 Traits of observed index	最小值/% Min	最大值/% Max	平均值/% Mean	标准差 s	变异系数/% CV
相对株高 Relative plant height	9.40	86.44	45.37	0.15	32.63
相对SPAD值 Relative SPAD value	12.14	195.61	111.08	0.42	37.68
相对地上部分鲜质量 Relative above-ground fresh weight	8.85	64.85	40.69	0.13	31.12
相对地下部分鲜质量 Relative under-ground fresh weight	4.01	60.25	26.71	0.14	50.93
相对叶面积 Relative leaf area	1.96	54.49	25.66	0.12	46.57

染色体 Chromosome	基因 Gene	起始位置/bp Gene start	终止位置/bp Gene end	功能 Function
Chr35448	LOC110917577	18787997	18791066	驱动蛋白轻链相关蛋白激酶
Chr35448	LOC110915949	18858783	18861543	铁硫簇组装蛋白
Chr35440	LOC110873143	79793745	79798128	铜-锌超氧化物歧化酶
Chr35447	LOC110922246	86212960	86215571	通用应激蛋白

染色体 Chromosome	基因 Gene	起始位置/bp Gene start	终止位置/bp Gene end	功能 Function
Chr35448	LOC110917577	18787997	18791066	驱动蛋白轻链相关蛋白激酶
Chr35448	LOC110915949	18858783	18861543	铁硫簇组装蛋白
Chr35440	LOC110873143	79793745	79798128	铜-锌超氧化物歧化酶
Chr35447	LOC110922246	86212960	86215571	通用应激蛋白

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