ACTA AGRICULTURAE BOREALI-SINICA ›› 2016, Vol. 31 ›› Issue (S1): 140-145. doi: 10.7668/hbnxb.2016.S1.025

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SSR Primers Development Based on Zijuancha Leaves' Transcription Group

ZHOU Meng1,2, SONG Weixi1,2, YAN Wenguang1,2, LI Xiaoxia1,2, CHEN Jiwei1,2, XIA Lifei1,2, LIANG Jiabin1,2, CHEN Linbo1,2   

  1. 1. Tea Research Institute, Yunnan Academy of Agricultural Sciences, Menghai 666201, China;
    2. Yunnan Technology Engineering Research Center of Tea Germplasm Innovation and Supporting Cultivation, Menghai 666201, China
  • Received:2016-06-28 Published:2016-12-28

Abstract: To develop SSR Markers from a rare tea variety Zijuancha,Zijuancha leaves' transcriptome which had the highest number of SSR repeats were used to design and select Zijuancha SSR Markers.In this study,designed primers from sequence of transcription group of Zijuancha's leaves,and got 87 primer pairs.Took DNA from Zijuancha and 3 other tea accessions,PCR by each primer,and analyzed in 1% agarose gel electrophoresis.Results showed 63 primer pairs could amplify bands,with the effective amplification rate of 72.4%.33 primer pairs demonstrated the polymorphic characteristics,accounting for 52.4% of all effective primer pairs.According to this study,to designed primer pairs from sequence of Zijuancha transcription group was viable.To picked up specific primers of Zijuancha from those 63 primer pairs will be performed in the next study.

Key words: Zijuancha, SSR, Primer development

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Cite this article

ZHOU Meng, SONG Weixi, YAN Wenguang, LI Xiaoxia, CHEN Jiwei, XIA Lifei, LIANG Jiabin, CHEN Linbo. SSR Primers Development Based on Zijuancha Leaves' Transcription Group[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(S1): 140-145. doi: 10.7668/hbnxb.2016.S1.025.

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