QTL Primary Mapping of Cold Tolerance Related Genes in Brassica napus

doi:10.7668/hbnxb.2016.05.003

Abstract

Abstract: The low temperature is a major cause of yield loss in Brassica production.The object of this study was to understand the genetic causes underlying cold tolerance and to identify relevant quantitative trait locus.An F₂ population(147 individuals) derived from GZ hui(Brassica napus,high cold tolerance) and 10B(Brassica napus,low cold tolerance) were used as gene mapping population.The two parents and F_2:3 families were used as secondary research material.SSR molecular markers in combination with physiological index and natural conditions of hardiness survey were developped to map QTLs associated with cold tolerance genes.The results showed that 160 SSR primers could amplify polymorphic bands between the two parents among 630 SSR primers,the polymorphism rate was 25.40%;102 SSR primers showed polymorphism between F₂ among 160 SSR premiers with polymorphism rate was 63.75%.A total of 229 loci were finally identified.The genetic map covered 19 linkage groups with 974.70 cM in length,and average destiny was 5.70 cM.Under the condition of natural wintering,relative conductivity,relative water content,SPAD and MDA were measured in F₂ and F_2:3,respectively,and the correlation was analyzed among them showing correlation or significant correlation which indicated these indexes could be regarded as targets to evaluate the cold tolerance in Brassica.Five QTLs were identified associated with cold resistance.There was an overlap area between the QTLs of SPAD and MDA,which could be regarded as a hot area of candidate genes.This research can lay further foundation for fine mapping and cloning of the cold tolerance genes in Brassica napus.

Key words: Brassica napus, Cold tolerance, SSR markers, QTL mapping

CLC Number:

Q78
S635.03

ZHAO Na, LANG Lina, LU Hong, LIU Lu, LIU Yaping, LIU Xia, TIAN Zhengshu, WANG Yang, HUANG Zhen, XU Aixia. QTL Primary Mapping of Cold Tolerance Related Genes in Brassica napus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(5): 14-20. doi: 10.7668/hbnxb.2016.05.003.

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