Molecular Identification of Very Virulent Infectious Bursal Disease Virus

doi:10.3969/j.issn.1000-7091.2013.03.042

Abstract

Abstract: The infectious bursal disease virus VP2 gene was amplified from bursa tissues of infected chickens in different areas of Shanxi province by RT-PCR，and then cloned to pMD18-T vector for sequence analysis． The results revealed that the SX/12 VP2 gene contained 1 356 nucleotides and encoded 452 amino acid． Compared with those other stains from GenBank， the SX/12 VP2 shared 99． 2% homology with other very virulent infectious bursal disease virus( vvIBDV) strains at nucleotide level and 99． 6% homology at amino acid level． The phylogenetic analysis showed that SX/12 was in the same lineage with the vvIBDV reference strains． The key amino acid in hypervariable region except 222(S) represented the typical feature of those of vvIBDV strains at the positions of 249( Q) ，254( G) ， 256( I) ，2 79(D)，284(A)，294(I)，299(S) and SWSASGS at positions of 326-332． These results demonstrated that SX/12 was classified to be vvIBDV．

Key words: Infectious bursal disease virus, VP2 gene, Molecular identification

CLC Number:

S852.65

Zhan Lie, Lu Bingyang, Liu Huadong, Wang Caixian, Tang Juan, Zhan Haijie1, Li Qin, Wang Jianzhong, Ding Zhuang. Molecular Identification of Very Virulent Infectious Bursal Disease Virus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(3): 234-238. doi: 10.3969/j.issn.1000-7091.2013.03.042.

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http://www.hbnxb.net/EN/Y2013/V28/I3/234

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